miRNA-769-5p在乙型肝炎相关肝细胞癌中的表达  

Expression of MicroRNA-769-5p in Hepatitis B Virus-related Hepatocellular Carcinoma

作　　者：朱海涛[1] 董琼珠[2] 贾户亮[2] 钦伦秀[2] 

机构地区：[1]贵阳医学院附院肝胆外科,贵州贵阳550004 [2]复旦大学附属中山医院,上海200032

出　　处：《贵阳医学院学报》2012年第1期9-13,19,共6页Journal of Guiyang Medical College

基　　金：国家自然科学基金资助项目(30700991)

摘　　要：目的:研究乙型肝炎相关性肝细胞癌中异常表达的微小RNA(miRNAs)及其与肝细胞癌患者根治术预后的关系。方法:通过Taqman低密度芯片(TLDA)分析了显微切割福尔马林固定石蜡包埋(FFPE)样本中的肝细胞癌组织及癌旁组织中miRNA的表达,并在48例样本中验证了其中1个差异表达的miRNA(miR-769-5p)及与肝细胞癌患者根治性切除术预后的关系。结果:miR-769-5p在癌组织较癌旁组织中高表达,在48例显微切割的有、无早期复发肝细胞癌样本中验证了该结果并分析了miR-769-5p与肝细胞癌患者根治术预后的关系,结果发现miR-769-5p在早期复发者的癌组织中表达高于无早期复发者。但是,肿瘤中miR-769-5p的表达水平与复发时间及总生存时间无关。进一步分析发现miR-769-5p的表达可能与肿瘤的数目相关,而与肿瘤大小、包膜及肿瘤的分期无关。结论:miR-769-5p在乙型肝炎相关肝细胞癌组织中较癌旁组织高表达,且其表达水平可能与肿瘤的数目相关。Objective： To investigate aberrantly expressed microRNA （miRNA） in hepatitis B virus- related hepatocellular carcinoma （HCC） and its relation with prognosis of HCC patients after radical resection. Methods： Taqman low density array （TLDA） was employed to analyze miRNA expression in tumor tissues and its adjacent tissues in sections of formalin-fixed and paraffin-embedded （FFPE）HCC specimens. A differentially expressed microRNA （miR-769-Sp） in 48 samples was validated, and its relationship with prognosis of HCC patients after radical resection was analyzed. Results： miR- 769-5p was found highly expressed in tumor tissues compared with adjacent tissues. This result was validated in 48 HCC specimens; Furthermore, miR-769-Sp expression level in tumor tissues of earlyrecurrence group was higher than that in non-early recurrence group. Tumor miR-769-5p levels were not found associated with recurrence time or overall survival time of HCC patients, miR-769-5p might be associated with tumor number, but not tumor size, encapsulation, or tumor stages. Conclusions： miR-769-5p is upregulated in HCC tissues compared with adjacent tissues, and its expression may be associated with tumor number.

关 键 词：微小RNA 癌 肝细胞 肝炎 乙型 

分 类 号：R575.1[医药卫生—消化系统]