FOXP3基因多态性与原发性肝细胞癌  

FOXP3 gene polymorphisms and hepatocellular carcinoma

作　　者：陈衍辉[1] 张恒辉[1] 王艳[2] 廖维甲[2] 覃理灵[2] 谢兴旺[1] 费然[1] 王雪艳[1] 梅铭惠[2] 魏来[1] 陈红松[1] 

机构地区：[1]北京大学人民医院北京大学肝病研究所北京市丙型肝炎和肝病免疫治疗重点实验室,100044 [2]桂林医学院附属医院

出　　处：《中华普通外科杂志》2012年第2期127-130,共4页Chinese Journal of General Surgery

基　　金：基金项目：国家自然科学基金资助项目（91029741,81001072）;“十一五”科技重大专项基金资助项目（2008ZXl0002-008,2008ZXl0002-019）

摘　　要：目的探讨转录因子FOXP3基因单核苷酸多态性（singlenucleotidepolymorphisms，SNPs）与原发性肝细胞癌（hepatocellularcarcinoma，HCC）的关系。方法采用飞行时间质谱法（MALDI－TOF）对392例肝癌患者和372例健康对照者FOXP3基因rs2280883和rs3761549位点单核苷酸多态性进行分析。结果肝癌组FOXP3基因SNP位点rs3761549C等位基因的频率明显高于健康对照组（OR=1．32，95％C／1．03～1．70，P=0．027）。肝癌患者FOXP3基因在rs2280883位点上出现TT基因型和在rs3761549位点上出现CC基因型的比例较健康对照者高，分别为79．6％和77．6％，并且携带rs2280883TT基因型或者rs3761549CC基因型的患者发生肝癌的风险也较高（OR=1．53，95％C／1．10～2．14；OR=1．92，95％C／1．39—2．64；P〈0．001）。分层分析肝癌患者的临床资料发现，rs3761549位点上CC基因型与原发性肝细胞癌发病的关联在发生门静脉癌栓的患者中更加显著（X^2=5．578，P=0．018），而且rs3761549位点上TT／CT基因型与原发性肝细胞癌发病的关联在出现肝癌复发的患者中更加显著（0=6．561，P=0．010）。结论FOXP3基因rs2280883和rs3761549位点的多态性与原发性肝细胞癌发病具有相关性，其中rs3761549位点CC基因型和TT／CT基因型分别与原发性肝细胞癌患者的门静脉癌栓发生和肝癌复发具有相关性。Objective To investigate the correlation between single nucleotide polymorphisms （SNPs） of FOXP3 gene and the susceptibility of hepatocellular carcinoma （HCC）. Methods Two SNPs rs2280883 and rs3761549 of FOXP3 gene in 392 HCC patients and 372 healthy controls were analyzed by Matrix-Assisted Laser Desorption/ Ionization Time of Flight Mass Spectrometry （MALDI-TOF）. Results At rs3761549, C allele frequency was significantly higher （ OR = 1.32, 95% CI 1.03 - 1.70, P = 0. 027 ） in HCC patients than healthy controls. Compared with healthy controls, HCC patients had higher frequencies of TT genotype （ 79. 6% ） at rs2280883 or CC genotype （ 77.6% ） at rs3761549 of FOXP3 gene. Patients carrying rs2280883 TT genotype （ OR = 1.53, 95% CI 1.10 - 2. 14, P 〈 0. 00001 ） or rs3761549 CC genotype （ OR = 1.92, 95% CI 1.39 - 2. 64, P 〈 0. 00001 ） were more susceptible to HCC. Stratified analysis showed that rs3761549 CC genotype was significantly associated with higher incidence of portal vein tumor thrombus （ x^2 = 5. 578, P = 0. 018 ）, and rs3761549 TT/CT genotype was significantly associated with higher rate of tumor recurrence in HCC patients （X^2 = 6. 561, P = 0.010 ）. Conclusions FOXP3 gene polymorphisms at rs2280883 and rs3761549 might be associated with increased susceptibility to HCC. rs3761549, CC genotype and TT/CT genotype were respectively associated with higher incidence of portal vein tumor thrombus and tumor recurrence in HCC patients.

关 键 词：癌 肝细胞 多态性 单核苷酸 FOXP3基因 

分 类 号：R735.7[医药卫生—肿瘤]