大黄素对P2X_(2/3)受体介导的三叉神经痛的作用研究  

The role of emodin in trigeminal neuralgia mediated by P2X_(2/3) receptor

作　　者：吴饶平[1] 熊伟[2] 欧晓艳[2] 刘晗[1] 邱淑怡[1] 李桂林[1] 徐昌水[1] 刘双梅[1] 梁尚栋[1] 高云[1] 

机构地区：[1]南昌大学基础医学院生理学教研室,江西南昌330006 [2]南昌大学附属口腔医院预防科,江西南昌330006

出　　处：《中国药理学通报》2012年第3期326-332,共7页Chinese Pharmacological Bulletin

基　　金：国家自然科学基金资助项目(No 30860333;81100829);江西省卫生厅课题资助(No 20101066);江西省青年科学家培养计划资助课题(2010)

摘　　要：目的探究大黄素(emodin)对P2X2/3受体介导的三叉神经痛的作用影响。方法慢性压迫性损伤大鼠眶下神经(CCI-ION)建立大鼠三叉神经痛动物模型,应用von Frey细丝法测试机械痛敏阈值,观察大黄素对三叉神经痛(TN)大鼠机械痛敏阈值的影响。结合原位杂交、PT-PCR、免疫组织化学、免疫荧光、蛋白印迹等方法观察大鼠三叉神经节(TG)中P2X2/3受体的表达变化。结果术后14 d,Ⅲ组(TN模型组)和Ⅰ组(生理盐水对照组)、Ⅱ组(假手术组)、Ⅳ组(TN模型+大黄素干预组)相比较,大鼠手术侧眶下神经面部感觉区域机械痛敏阈值明显降低(P<0.01),大鼠TG中P2X2/3受体表达明显升高(P<0.01);Ⅰ、Ⅱ、Ⅳ组之间相比,大鼠手术侧眶下神经面部感觉区域机械痛敏阈值差异没有显著性(P>0.05);Ⅳ组TG中P2X2/3受体的表达较Ⅲ组低(P<0.01)。结论 P2X2/3受体涉及到三叉神经痛,大黄素对P2X2/3受体介导的三叉神经痛有一定抑制作用。Aim To explore the role of emodin in trigeminal neuralgia mediated by P2X2/3 receptor.Methods The trigeminal neuralgia rat model（a chronic constriction injury of the infraorbital branch of the trigeminal nerve,ION-CCI） was established.The mechanical hyperalgesia threshold of trigeminal neuralgia（TN） rats was tested by the von Frey filaments.The change of the expression of P2X2/3 receptors in rat trigeminal ganglion（TG） was detected with in situ hybridization,RT-PCR,immunohistochemical staining,immunofluorescence,and Western Blot.Results 14 days after operation,compared withⅠ group（saline control group）,Ⅱ group（sham operation group） or Ⅳ group（TN model＋emodin intervention group）,Ⅲ group（TN model group） had a significant decrease of mechanical hyperalgesia threshold in the territory of ligated infraorbital nerve（P0.01）,and an obvious increase in expression of P2X2/3 receptor in rat TG（P0.01）;there was no significant differences of mechanical hyperalgesia threshold amongⅠ,Ⅱ and Ⅳ group（P0.05）;the expression of P2X2/3 receptor of TG in Ⅳ group was lower than that in Ⅲ group（P0.01）.Conclusion P2X2/3 receptor may play a key role in the pain transmitting of trigeminal neuralgia,and emodin has inhibitory effect on trigeminal neuralgia.

关 键 词：P2X2受体 P2X3受体 三磷酸腺苷 大黄素 三叉神经痛 治疗 

分 类 号：R-332[医药卫生] R282.71