应用胚胎干细胞试验模型对碱性成纤维细胞生长因子发育毒性的初步评价  

Embryonic stem cell test for the study of the developmental toxicity of basic fibroblast growth factor

作　　者：许华[1,2] 邓淑琴[1] 何清[1] 苏本金[1] 江海香[1] 黄亚东 

机构地区：[1]暨南大学药学院,广东广州510632 [2]暨南大学中药药效物质基础及创新药物研究广东省高校重点实验室,广东广州510632 [3]医药生物技术研究开发中心,广东广州510632

出　　处：《中国药理学通报》2012年第3期347-351,共5页Chinese Pharmacological Bulletin

基　　金：国家自然科学基金资助项目(No 30973602);广东省科技计划项目(No 2011B031700009);广州市科技计划项目(No 2010Y1-C851)

摘　　要：目的利用小鼠胚胎干细胞试验(EST)模型,初步评价bFGF的发育毒性。方法体外培养小鼠胚胎干细胞(ESC),通过形态学观察、碱性磷酸酶染色和RT-PCR方法进行ESC未分化鉴定;MTT法检测不同浓度bFGF对ESC和3T3细胞的毒性,RT-PCR半定量分析法检测bFGF对未分化基因Sox-2表达的影响。结果 bFGF对ESC和3T3的半数抑制浓度和ESC的半数抑制分化浓度分别为IC50ESC=15.2 mg.L-1,IC50 3T3=24.2 mg.L-1,ID50 ESC=1.7 mg.L-1。结论 bFGF发育毒性的判定为弱胚胎毒性。Aim To research the developmental toxicity of basic Fibroblast Growth Factor（bFGF） by the embryonic stem cell test.Methods Mouse embryonic stem cells were cultured and their undifferentiations were identified by the methods of morphology,alkaline phosphatase staining and RT-PCR.The cytotoxic effect of bFGF was detected by MTT assay.The effect of bFGF on Sox-2 gene expression was determined by semi-quantitative RT-PCR.Result The IC50 of bFGF on 3T3 was 24.2 mg·L-1,while IC50 and ID50 on ESC were 15.2 mg·L-1 and 1.7 mg·L-1 respectively.Conclusion The developmental toxicity of bFGF is evaluated as weak embryotoxicity.

关 键 词：小鼠胚胎干细胞 胚胎干细胞试验模型 碱性成纤维细胞生长因子 Sox-2胚胎毒性 发育毒性 

分 类 号：R-332[医药卫生] R329.24