小鼠局灶性脑缺血模型行为学指标相关性研究  

Correlation between behavioral indicators in a mouse model of focal cerebral ischemia

作　　者：刘家兰[1] 邓礼娟[1] 原欢欢[1] 王琴[1] 徐晓玉[1] 

机构地区：[1]西南大学药学院,重庆市药效评价工程技术研究中心,重庆400715

出　　处：《中国药理学通报》2012年第3期430-435,共6页Chinese Pharmacological Bulletin

基　　金：重庆市科技攻关项目(No CSTC,2010AC5011);西南大学研究生创新基金(No Ky20091010)

摘　　要：目的探讨小鼠局灶性脑缺血模型神经功能指标间的相关性。方法采用线栓法制备局灶永久性小鼠脑缺血模型,选取不同的神经功能行为学指标并测定梗死质量百分率,对模型损伤程度进行评分,获得指标间的相关系数;选取葛根素、依达拉奉验证上述指标是否可用于药物筛选的研究。结果术后脑缺血小鼠神经行为学与神经综合功能评分、爬板试验和转角试验相关;脑梗死质量百分比与神经综合功能评分、爬板试验相关,与转角试验不具相关性。葛根素、依达拉奉均明显降低所选指标分值,抑制小鼠的脑缺血损伤。结论以上指标可用于评估药物对小鼠脑缺血损伤的影响研究。Aim To explore the correlation between different indicators of neurological function in mice after focal cerebral ischemia model（MCAO）.Methods Suture method was used to produce permanent focal cerebral ischemia model.Different neurological function indicators were selected and percentage of brain infarction was measured to evaluate the damage degree of model.Correlation coefficient between indicators were obtained.Puerarin and Edaravone were used to validate the rationality of chosen indicators.Results There was significant correlation between neurological behavioral and other neurological function indicators after operation.Cerebral infarction size percentage was significantly correlated with neurological comprehensive function and inclined test except corner test.Puerarin and Edaravone significantly inhibited the cerebral ischemia injury in mice.Conclusion The above indicators can be used to assess the effects of drugs on cerebral ischemia in mice.

关 键 词：模型 小鼠 永久性脑缺血 行为学指标 相关性 脑梗死 葛根素 

分 类 号：R-332[医药卫生] R322.81