低强度脉冲超声波对Beagle犬牙周炎组织修复效应的初步研究  

Effect of Low-intensity Pulsed Ultrasound on Periodontitis in Beagle Dogs

作　　者：董妮[1] 宋锦璘[1] 冯格[1] 刘珉懿[1] 邓锋[1] 

机构地区：[1]重庆医科大学附属口腔医院正畸科,重庆401147

出　　处：《四川大学学报（医学版）》2012年第2期183-186,共4页Journal of Sichuan University(Medical Sciences)

基　　金：国家自然科学基金(批准号30870754);重庆市发展和改革委员会高技术产业重大产业技术开发项目(渝发改技[2007]1110号);重庆卫生局科研项目(2008-2-254)和重庆卫生局科研项目(2010-2-228)资助

摘　　要：目的探讨低强度脉冲超声波(LIPUS)对Beagle犬牙周炎组织的初步修复效应。方法采用牙槽骨缺损联合弹性结方法制备慢性牙周炎的Beagle犬动物模型,在相同常规治疗基础上选用LIPUS不同强度(50mW/cm2、100mW/cm2)脉冲波和50mW/cm2连续波治疗(频率1.5MHz、调制信号波宽200μs、重复率1kHz),共治疗8周(1次/d,20min/次),并进行牙周临床指标检测与组织学切片检查。结果 LIPUS治疗8周后,治疗组牙龈组织肿胀减轻,菌斑指数(PLI)、牙龈指数(GI)、牙周探针深度(PD)均较对照组低(P<0.05),未发现明显的牙龈退缩、附着丧失和根分叉病变等;组织学切片检查发现LIPUS治疗组牙龈组织黏膜上皮增生不明显,有丰富的胶原纤维生成,牙槽骨组织中骨吸收陷窝减少,成骨细胞较活跃地排列在新骨周围。结论 LIPUS具有潜在的牙周炎组织修复效应,应进一步考虑LIPUS在引导组织再生术(GTR)治疗中的辅助修复效应。Objective To investigate the effect of low intensity pulsed ultrasound（LIPUS） on periodontitis in Beagle dogs.Methods The animal model of chronic periodontitis was established with elastic ligature-induced alveolar bone defect in Beagle dogs.LIPUS with different intensity（50 mW/cm2,100 mW/cm2） pulse wave and 50 mW/cm2 continuous wave,1.5 MHz of frequency,200 μs of pulse width modulated signal,and 1 kHz of repetition rate were applied to the dogs for 8 weeks（once a day and 20 minutes every time）.The periodontal clinical examinations and histological biopsy were performed.Results With increased exposure to LIPUS,gum tissue swelling of the dogs was reduced.The plaque index（PLI）,gingival index（GI）,pocket depth（PD） of the treatment groups were lower than the control groups（P0.05）.No significant gingival recession,attachment loss or furcation was found. The histological examination revealed that there was no obvious gingival hyperplasia in epithelial tissues,but there were significant activities in relation to collagen formation,decrease of bone resorption,and generation of active osteoblasts.Conclusion LIPUS may help repair periodontal tissues and should be considered in guided tissue regeneration（GTR）.

关 键 词：低强度脉冲超声 牙周炎 BEAGLE犬 修复效应 

分 类 号：R781.4[医药卫生—口腔医学]