食管癌细胞株高侵袭力亚系的建立及其生物学特性研究  

Establishment and characterization of esophageal squamous cell carcinoma cell line subpopulation with higher invasive ability

作　　者：夏宇[1] 李秀娟[2] 张志强[1] 温浩[3] 胡翠梨[1] 

机构地区：[1]新疆医科大学第一附属医院消化科,新疆乌鲁木齐830054 [2]新疆医科大学病理生理学教研室,新疆乌鲁木齐830054 [3]新疆医科大学第一附属医院肝胆外科,新疆乌鲁木齐830054

出　　处：《中华肿瘤防治杂志》2012年第1期6-9,共4页Chinese Journal of Cancer Prevention and Treatment

基　　金：国家自然科学基金(81001101);新疆维吾尔自治区自然科学基金(2010211B20)

摘　　要：目的:建立具有不同转移潜能的高侵袭能力食管癌细胞株亚系并研究其生物特性。方法:利用Transwell侵袭小室从人食管鳞癌Eca-109细胞株筛选高侵袭能力食管癌亚系,HE染色比较细胞形态;四甲基偶氮唑蓝(MTT)法检测细胞增殖能力的变化;流式细胞术(FCM)检测细胞周期;蛋白质印迹法检测与侵袭能力相关基质金属蛋白酶-2(MMP-2)和金属蛋白酶组织抑制因子-2(TIMP-2)蛋白表达。结果:利用Transwell侵袭小室从食管癌Eca-109细胞株中筛选出高侵袭能力食管癌细胞株亚系,命名为Eca-109T4。2个细胞系细胞形态没有明显差异。MTT法检测显示,亚系Eca-109T4增殖能力强。细胞周期显示,增殖指数(PI)高,PI=41.0%,且MMP-2蛋白表达相比增高,P=0.023;TIMP-2蛋白表达相比增高,但差异无统计学意义,P=0.392。结论:建立了不同转移潜能的高侵袭能力食管癌细胞株亚系,将可以用于探讨食管癌转移机制的研究。OBJECTIVE： To establish a higher invasive ability cell line from esophageal squamous cell carcinoma cell line （ESCC）. METHODS： Transwell invasion chamber was used to subdivide a human esophageal carcinoma cell line named Eca-109 to sublines. The cell morphology were compared by HE staining; Methyl thiazolyl tetrazolium （MTT） method were used to detect changes in cell proliferation; the alteration of the cell cycle was detected using flow cytometry （FCM） ; The expression of invasion-related MMP-2 and TIMP-2 protein were detected by westen bloting. RESULTS：An esophageal squamous cell carcinoma subline was established and named Ecaq09 T4. There was no significant difference in cell morphology, MTT assay showed a strong proliferation of subline, and cell cycle showed proliferation index （PI） was 41.0~ ,Which was higher than Eca-109; Westen bloting showed that the expression of MMP 2 protein increased （P O. 023） ; TIMP-2 protein expression also increased,but the difference was not statistically significant （P=0. 392）. CON- CLUSION：The higher invasive ability of esophageal carcinoma cell line is obtained and may be used to further research works.

关 键 词：食管肿瘤 细胞系 肿瘤 肿瘤侵润 

分 类 号：R735.1[医药卫生—肿瘤]