外源性锌离子对大鼠海马切片癫痫样放电的起源、传播与频率特性的调节作用  

The Modulatory Effects of Exogenous Zn^(2+) on Propagation and Frequency of Epileptiform Discharges on Rat Hippocampal Slices

作　　者：李静波[1] 陆钦池[1] 宫新伟[2] 龚海庆[2] 张溥明[2] 梁培基[2] 

机构地区：[1]上海交通大学医学院附属仁济医院神经内科,上海200127 [2]上海交通大学生物医学工程系,上海200240

出　　处：《生物物理学报》2012年第2期119-130,共12页Acta Biophysica Sinica

基　　金：上海市自然科学基金项目(11ZR1421800);上海交通大学"医工(理)交叉研究基金"项目(G08PETZD05)

摘　　要：本研究采用多电极记录技术,在离体条件下研究外源性锌离子(Zn2+)对无镁人工脑脊液诱导的Sprague-Dawley大鼠海马切片癫痫样放电的起源、传播与频率特性的调节作用。结果表明:1μmol/L和100μmol/L的Zn2+作用于海马切片,不改变海马切片上癫痫样放电的起始位置,但能够降低癫痫样放电顺行和逆行两个方向的传播速度,并改变癫痫样放电不同频率范围成分所占的比例。以上结果提示,1μmol/L和100μmol/L的Zn2+可以对海马切片上的癫痫样放电起到调节作用,减慢癫痫样放电在网络中的传播速度,同时,可能对神经元放电活动起到去同步化的作用。The modulatory effects of exogenous Zn2＋ on initiation,propagation and frequency characteristics of epileptiform discharges induced by Mg2＋-free artificial cerebrospinal fluid（ACSF） in hippocampal slices of Sprague-Dawley rats were examined by multi-electrode array（MEA） system.The main results of the present study are： the modulatory effects of 1 μmol/L and 100 μmol/L Zn2＋ did not change the initiation site of the epileptiform discharges,but the antegrade and retrograde speed of epileptiform discharges were slowed down by perfusion of Zn2＋,and the ratio of different frequency components were changed by Zn2＋ application.These results imply that 1 μmol/L and 100 μmol/L Zn2＋ play the neuromodulatory effects on epileptiform discharges in hippocampal slices,which can slow down the propagation speeds of epileptiform discharges in the network.Zn2＋ may also play the desynchronization effects on the neural firing activity.

关 键 词：癫痫样放电 锌离子 微电极阵列 海马切片 

分 类 号：R742.1[医药卫生—神经病学与精神病学]