血根碱对卵巢癌细胞生长及放射敏感性的影响  

Effect of sanguinarine on the growth and radiosensitivity of human ovarian cancer cells

作　　者：徐加英[1] 吉俊敏[2] 焦旸[1] 伍理[1] 樊赛军[1] 

机构地区：[1]苏州大学医学部放射医学与公共卫生学院肿瘤放射生物学重点实验室,215123 [2]常州市疾病预防控制中心

出　　处：《中华放射医学与防护杂志》2012年第1期6-10,14,共6页Chinese Journal of Radiological Medicine and Protection

基　　金：教育部“长江学者和创新团队发展计划资助”项目（IRT0849）;江苏省普通高校研究生科研创新计划（CX10B-0452）;苏州大学优秀博士学位论文选题立项（233209749）

摘　　要：目的探讨血根碱对卵巢癌SK-OV-3细胞的生长及放射敏感性的影响。方法采用MTT法和克隆形成法检测血根碱处理后卵巢癌SK—OV-3细胞的生长；流式细胞仪分析方法观察血根碱对细胞周期分布和细胞凋亡的影响；AnnexinV／PI法观察血根碱联合辐照对细胞凋亡的影响。结果0—5μmol／L血根碱处理24和48h后，细胞生长的抑制与血根碱处理的剂量和时间成正相关（r=0．96和0．97），半数细胞生长抑制的浓度（IC50）分别为3．02和1．11μmol／L。血根碱处理导致了包括细胞变圆、皱缩，漂浮细胞增多等细胞形态学的改变。Sub．G，凋亡峰随血根碱浓度上升而增高，并出现G0／G1期细胞周期阻滞。0．5μmol／L血根碱预处理24h后经6Gy的x射线辐照，早期凋亡细胞从10．28％增加至43．28％（t=19．41，P〈0．01），晚期凋亡细胞从20．26％增加至30．80％（t=8．78，P〈0．01）。采用多靶单击模型拟合曲线发现血根碱＋X射线组与X射线单独照射组的放射增敏比（SERD。）达1．625，即低浓度的血根碱可以增加卵巢癌细胞的放射敏感性。结论血根碱可以显著抑制卵巢癌SK-OV-3细胞的生长，诱导细胞凋亡和周期阻滞，而且在低剂量下，血根碱可以增加卵巢癌细胞的放射治疗敏感性。Objective To study the effect of sanguinarine on the growth and radiosensitivity of ovarian cancer SK-OV-3 cells. Methods Cell growth was determined by MTT and clonogenic assay. Cell cycle analysis was performed by flow cytometry assay. The cell apoptosis was analyzed by Annexin V/PI assay. Results Sanguinarine inhibited SK-OV-3 cell growth in a dose-and time-dependent fashion and its IC50 values were 3.02 and 1.11 μmol/L at 24 and 48 h, respectively. Sanguinarine also significantly triggered a sub-G1 peak, an indicator of apoptosis, and caused a G0/G1 arrest. Furthermore, the cell apoptosis induced by X-irradiation was significantly increased at 6 Gy when the cells were pre-treated with sanguinarine, in which the early apoptotic population increased from 10.28% to 43.28% （t = 19.41, P 〈 O. 01 ） and the late apoptotic population increased from 20. 26% to 30. 80% （ t = 8.78, P 〈 0.01 ）. The multi-target click model was used to fit survival curves and the SER of sanguinarine treatment approached to 1. 625 at the dose of Do. Conclusions Sanguinarine could inhibit SK-OV-3 cell growth by inducing apoptosis and cell cycle arrest and enhance cell radiosensitivity at low doses.

关 键 词：血根碱 卵巢癌 细胞周期 凋亡 放射增敏性 

分 类 号：R737.31[医药卫生—肿瘤]