磷酸化蛋白质（多肽）组学分析与筛查急性白血病特异性分子标志  

Screen of phosphopeptide specific for acute leukemia

作　　者：彭宏凌[1] 戴崇文[1] 张广森[1] 顾挺磊 余健 

机构地区：[1]中南大学湘雅二医院血液科,长沙410011 [2]美国细胞信号公司

出　　处：《中华血液学杂志》2012年第3期163-168,共6页Chinese Journal of Hematology

摘　　要：目的筛查与分析急性白血病特异性的磷酸化蛋白质或多肽。方法对16例初治急性淋巴细胞白血病（ALL）和20例急性髓系白血病（AML）患者骨髓单个核细胞行蛋白多肽抽提及纯化，采用免疫沉淀及结合高效液相质谱-串联质谱分析，筛查特异性分子标志物。结果①非受体型酪氨酸蛋白激酶家族的Fyn、Yes和Src等在急性白血病中广泛表达；②非受体型酪氨酸蛋白激酶家族的Abl／isol、Abl，非受体型丝氨酸／苏氨酸蛋白激酶家族的Bcr、JNK2、JNK2is02，结合器／支架类的Cas—L、Cbl、CrkLCENTD1（Centaurin deltal）ZO2，转录调节因子GFR—1及磷酸酯酶SHIP-2，这些磷酸化的蛋白见于Ph染色体阳性ALL，基本不表达于其他ALL；③Hck、Lyn和Fgr选择性地与AML相关（M3除外）。结论ALL和AML存在相对特异的磷酸化多肽，为急性白血病的诊断及治疗提供了潜在的分子靶位。Objective To screen phosphopeptide specific for acute leukemia. Methods Mononuclear cells from bone marrow were collected from 16 newly diagnosed acute lymphoblastic leukemia （ALL） and 20 acute myeloid leukemia （AML） patients. Peptides were extracted and purified, analyzed by immunoprecipitation and liquid chromatography coupled with tandem mass spectrometry （LC-MS）. Results （1）Non-receptor tyrosine kinase family members Fyn, Yes, Src widely expressed in acute leukemia; （2） Some phosphopeptides, including non-receptor tyrosine kinase family members Abl/isol and Abl, non-receptor Ser/Thr protein kinase family members Bcr, JNK2, JNK2 iso2, Adaptor/scaffold members Cas-L, Cbl, CrkL CENTD1 （Centanrin deltal ） ZO2, transcriptor GFR-1 and phosphatase SHIP-2 were detected in Ph positive ALL, but not in other kinds of ALL. （3）Hck, Lyn and Fgr selectively expressed in AML （ except AML-M3 ）. Conclusion Some phosphopeptides were specific for ALL and AML, and may be useful for diagnosis and therapy of acute leukemia.

关 键 词：白血病 磷酸化多肽 免疫沉淀 磷酸化蛋白质组 高效液相质谱-串联质谱 

分 类 号：R733.7[医药卫生—肿瘤]