自体CIK细胞联合IL-2治疗难治性脾非霍奇金淋巴瘤1例  

Treatment of refractory non-Hodgkin's lymphoma of the spleen with combined autologous cytokine induced killer cells and IL-2: A case report

作　　者：杨洋[1] 杨波[1] 脱帅[2] 卢学春[1] 朱宏丽[1] 脱朝伟[2] 蔡力力[1] 迟小华 于睿莉[1] 陈云燕 张文英[1] 刘洋[1] 王瑶[1] 代汉仁[1] 韩为东[1] 张峰[5] 姚善谦[1] 

机构地区：[1]解放军总医院,北京100853 [2]解放军第202医院,沈阳110003 [3]解放军第二炮兵总医院药学部,北京100800 [4]解放军第二炮兵后勤部保健办,北京100800 [5]中国科学院北京基因组研究所,北京100029

出　　处：《军医进修学院学报》2012年第3期285-287,共3页Academic Journal of Pla Postgraduate Medical School

基　　金：国家自然科学基金项目(30772597);解放军总医院科技创新苗圃基金(11KMM24);中央保健研究基金(B2009B115);科技部新药创制重大专项(2008ZXJ09001-019)~~

摘　　要：目的观察自体细胞因子诱导的杀伤细胞输注联合IL-2治疗老年淋巴瘤的有效性和安全性。方法 1例老年脾脏恶性淋巴瘤患者在经过手术切除脾脏原发肿瘤、8个疗程的R-CHOP方案免疫化疗后出现淋巴瘤肝脏转移,此时采集患者外周血单核细胞,在体外经干扰素-γ(IFN-γ)、白介素-2(IL-2)和抗CD3单克隆抗体诱导成CIK细胞,每次回输细胞数为2-3×109个,回输后应用IL-2 100mU/d,皮下注射,连续10d,28d为1个周期。观察治疗前后患者肝功能、肿瘤相关生物学指标及影像学变化。结果患者共完成8个周期的CIK细胞输注,每次回输后未出现不良反应,肝功能指标、LDH水平均降至正常,PET/CT检查示治疗后肝脏淋巴瘤转移灶消失,达到完全缓解。结论自体CIK细胞输注联合IL-2疗法对于清除治疗后淋巴瘤的微小残留灶和(或)转移灶安全有效。Objective To observe the efficiency and safety of combined autologous cytokine induced killer（CIK） cells and IL-2 in treatment of splenic lymphoma in old patients.Methods Hepatic metastasis of splenic primary malignant lymphoma occurred in an old patient after splenectomy and 8 cycles of R-CHOP chemotherapy.Peripheral blood mononuclear cells（PBMC） were collected.CIK cells were induced with in vitro interferon gamma（IFN-γ）,IL-2 and anti-CD3 monoclonal antibody（mAb）.Liver function,tumor-related biological indexes and image changes were observed after 2-3×109 CIK cells were re-transfused into the patient each time and IL-2 100mU/d was subcutaneously injected for 10 days,28 days a cycle.Results No adverse reaction occurred in the patient after 8 cycles of CIK cells transfusion.The liver function and serum LDH level became normal（P0.05）.PET-CT showed that hepatic metastasis of lymphoma disappeared and completely relieved.Conclusion Combined autologous CIK cells and IL-2 is safe and effective for small residual or metastatic foci of lymphoma in old patients after splenectomy.

关 键 词：细胞因子诱导的杀伤细胞 脾脏淋巴瘤 老年人 

分 类 号：R733.42[医药卫生—肿瘤]