结肠癌β-catenin、Oct-4表达及其与术后复发转移的关系  

Expression of β-catenin and Oct-4 in colonal cancer and the relationship with metastasis afteroperation

作　　者：李宁[1] 邓文英[1] 马怡晖[2] 陈小兵[1] 陈贝贝[1] 罗素霞[1] 

机构地区：[1]河南省肿瘤医院内科,郑州450000 [2]郑州大学第一附属医院病理科

出　　处：《肿瘤研究与临床》2012年第1期4-7,共4页Cancer Research and Clinic

基　　金：基金项目：河南省医药卫生重大攻关项目卫生部科研基金项目（WKJ2007-2-026）;河南省社会公益项目（胃癌肿瘤干细胞因子检测及临床研究）

摘　　要：目的探讨结肠癌患者肿瘤组织中B．catenin、Oct-4表达及其对远处转移的预测作用。方法采用免疫组化方法检测80例结肠癌患者术后肿瘤组织中B-catenin、Oct-4蛋白表达情况，并分析其与肿瘤分化程度、分期和术后复发转移的相关性。采用RT-PCR检测20例冷冻标本肿瘤组织及癌旁组织中Oct-4mRNA表达情况。结果80例患者有35例出现复发转移，β-catenin、Oct-4在转移组患者中表达率分别为65．71％（23例）、51．43％（18例），在非转移组分别为31．11％（14例）、13．33％（6例），两组间阳性表达率差异均有统计学意义（x^2=9．843，P=0．002；x^2=13．605，P=0．001）。不同肿瘤分化程度、T分期和N分期者β-catenin、Oct-4表达差异均无统计学意义（P〉0．05）。肿瘤组织中Oct-4mRNA表达明显高于癌旁组织。β-catenin、Oct-4均为阳性表达者和均为阴性表达者术后远处转移率分别为78．57％（11／14）和12．50％（4／32），生存分析显示不同表达状态其远处转移出现时间差异有统计学意义（P〈0．05）。结论肿瘤组织中β-catenin、Oct-4表达同结肠癌术后转移的发生相关。Objective To investigate the expression of β-catenin and Oct-4 in colonal carcinoma and explore the relationship with recurrence and metastasis after operation. Methods Immunohistochemical analysis was used to evaluate the expression of β-catenin and Oct-4. The correlation of β-catenin and Oct-4 expression with tumor cell differentiation, T stage, N stage and metastasis was analyzed. The gene expression of Oct-4 was examined by RT-PCR in 20 frozen tumor tissues and normal tissues adjacent to tumor. Results Thirty-five patients had metastasis. The positive rates of β-catenin and Oct-4 expression were significantly higher in metastasis group than in the non-metastasis group （65.71% vs 31.11%, 51.43 % vs 13.33 %, X2 = 9.843, P = 0.002, X2 = 13.605, P = 0.001）. Expression of β-catenin and Oct-4 was not associated with differentiation, T stage or N stage. The positive expression rate of Oct-4 in colonal carcinoma tissues was significantly higher than that in normal tissues. Metastatic rates in patients with positive expression of β-catenin and Oct-4 was higher than that in negative expression. The survival analysis showed that time of metastasis was significantly different in two groups of patients （P 〈0.05）. Conclusion The expression of β-catenin and Oct-4 in tumor tissues is related to metastasis of colonal cancer after surgery and might be used to predict metastasis of colonal cancer after operation.

关 键 词：结肠肿瘤 肿瘤标记 生物学 Β-CATENIN Oct.4 转移 

分 类 号：R735.34[医药卫生—肿瘤]