乳腺癌组织中Ras相关C3肉毒菌毒物底物1和缺氧诱导因子1α蛋白的表达及其临床意义  

Expression and clinical significance of Rac-1 and HIF-lot in breast cancer

作　　者：毕益明[1] 付艳[2] 陈秀芳[1] 李新军[3] 陈元[4] 

机构地区：[1]山东省滨州市人民医院肿瘤二科,256610 [2]山东省滨州市人民医院皮肤科,256610 [3]山东省滨州市人民医院病理科,256610 [4]华中科技大学同济医学院附属同济医院肿瘤中心

出　　处：《肿瘤研究与临床》2012年第2期98-101,共4页Cancer Research and Clinic

摘　　要：目的探讨Ras相关c3肉毒菌毒物底物1（Rac-1）与缺氧诱导因子1d（HIF-1α）在乳腺癌中的表达及其与临床分期、组织学分级及激素受体状态的关系。方法乳腺癌标本共139例，应用免疫组织化学方法检测乳腺癌组织中Rac-1和HIF-1α蛋白的表达，并结合临床病理因素及激素受体状态进行统计学分析。结果Rac-1、HIF-1α在乳腺癌组织中的阳性率分别为64．75％（90／139）和65．47％（91／139），二者均与TNM分期及组织学分级有关（均P〈0．05），与年龄、性别、组织类型、月经状态无关（均P〉0．05）。Rac-1、HIF-1α在三阴性乳腺癌与非三阴性乳腺癌组织中表达差异无统计学意义（X2=1．1229，x2=0．1786，均P〉0．05）。在乳腺癌组织中，Rac-1与HIF-1α的表达呈正相关（L=0．414，P〈0．01）。结论Rac-1和HIF-1α在乳腺癌组织中的表达具有相关性，可能成为反映乳腺癌生物学行为的新型分子标志物，阻断二者之间的联系在乳腺癌的治疗中可能具有较高的临床意义。Objective To explore the expression and clinical significance of Ras-related C3 botulinum toxin substrate 1 （Rac-1） protein and hypoxia-inducible factor lot （HIF-1α） in breast cancer and their relationship with tumor stage, node metastasis status and hormone receptor status. Methods Immunohistochemical method was used to detect the expression of Rac-1 and HIF-1α protein in 139 specimens of breast cancer, combined with their clinical pathological characteristics and hormone receptor status for statistical analysis. Results The expression of Rac-1 and HIF-1α in breast cancer tissue were 64.75 % and 65.47 %, respectively. The levels were related with the stage of TNM and histological grade （P 〈 0.05, P 〈 0.01）. There was no significant correlation between these two proteins＂ expression and the age of patient, primary pathological location, histologic type and menstruation status of breast cancer patients （P 〉 0.05）. The expression of Rac-1 and HIF-1α had no statistical significance between triple negative breast cancer （TNBC） and non-triple negative breast cancer （NTNBC） patients （X2 = 1.1229, X3 = 0.1786, P 〉 0.05）; The expression level of Rac-1 was positively correlated with HIF-1α in these breast cancer specimens （rs = 0.414, P 〈 0.01）. Conclusion The expression of Rac-1 is positively correlated with HIF-1α level in breast cancer. The relationship between Rac-1 and HIF-1α might be as a new important marker to estimate the invasion, metastasis extent and prognosis in breast cancer. Blocking the regulation between Rac-1 and HIF-1α might be a new treatment target in breast cancer.

关 键 词：乳腺肿瘤 Ras相关C3肉毒菌毒物底物1 缺氧诱导因子1Α 免疫组织化学 

分 类 号：R737.9[医药卫生—肿瘤]