STAT3在食管上皮内瘤变及食管癌组织中的表达及其意义  

Expression of STAT3 in tissue of esophageal intraepithelial neoplasia and esophageal neoplasm and its significance

作　　者：刘惠民[1] 郭梅艳[2] 王彦华[1] 王蕾[1] 赵琰龙[2] 韩起廷[1] 瞿峰[2] 王智红[3] 王秀清[2] 

机构地区：[1]河北工程大学医学院病理教研室,河北邯郸056002 [2]河北工程大学附属医院乳腺外科,河北邯郸056002 [3]河北省邯郸市中心血站采血科,056002

出　　处：《重庆医学》2012年第7期649-650,653,共3页Chongqing medicine

基　　金：河北省科学技术研究与发展计划基金资助项目(11276103D-32);邯郸市科技局计划基金资助项目(1123108077-2)

摘　　要：目的探讨STAT3mRNA及STAT3蛋白在食管上皮内瘤变演变过程中的作用。方法选取食管癌高发区的食管上皮内瘤变病例150例(Ⅰ、Ⅱ、Ⅲ级各50例)及食管癌100例进行研究,将其中60例接受食管癌切除术患者的食管上、下残端的正常黏膜组织作为对照。采用原位杂交技术和免疫组织化学法检测正常食管黏膜、食管上皮内瘤变及食管癌组织中STAT3在mRNA及蛋白水平的表达情况。结果 STAT3mRNA在正常食管黏膜、食管上皮内瘤变及食管癌组织中的阳性表达率分别为3.33%、53.33%及89.00%;STAT3蛋白在上述组织中的阳性表达率分别为6.67%、60.67%及94.00%。不同程度的食管上皮内瘤变病例中,病变组织中STAT3mRNA及STAT3蛋白的表达随食管上皮内瘤变病变程度的加重而增加。STAT3在mRNA和蛋白水平的表达存在一致性(r=0.768,P<0.05)。结论 STAT3在食管上皮内瘤变及食管癌组织中高表达,它可能在食管上皮内瘤变的演变过程中发挥重要作用。Objective To explore the role of STAT3 mRNA and STAT3 protein in the evolution of esophageal intraepithelial neoplasia.Methods 150 cases of esophageal intraepithelial neoplasia（gradeⅠ,Ⅱ and Ⅲ with 50 cases in each） and 100 cases of esophageal neoplasm in area with high incidence of esophageal neoplasia were selected.The normal mucosal tissue in upper and lower esophageal stump of 60 patients among them who had undergone esophageal carcinoma resection served as control.In situ hybri-dization and immunohistochemistry were employed to detect STAT3 expression at mRNA and protein levels in tissue of normal esophageal mucosa,esophageal intraepithelial neoplasia and esophageal neoplasm.Results The positive expression rates of STAT3 mRNA in tissue of normal esophageal mucosa,esophageal intraepithelial neoplasia and esophageal neoplasm were 3.33%,53.33% and 89.00%,respectively,and those of STAT3 protein in tissue above were 6.67%,60.67% and 94.00%,respectively.In different degrees of esophageal intraepithelial neoplasia cases,expressions of STAT3 mRNA and STAT3 protein in pathological tissue enhanced with severity of esophageal intraepithelial neoplasia increasing.There was consistency in STAT3 expression at mRNA and protein levels（r=0.768,P0.05）.Conclusion Highly expressed STAT3 in tissue of esophageal intraepithelial neoplasia and esophageal neoplasm probably plays important roles in evolution of esophageal intraepithelial neoplasia.

关 键 词：食管肿瘤 原位杂交 免疫组织化学 食管上皮内瘤变 信号转导子和转录激活子 

分 类 号：R735.1[医药卫生—肿瘤]