直肠癌组织β-catenin和β-Trcp表达及其临床意义的探讨  

Expression and clinical significance of β-catenin and β-Trcp in the generating process of the rectal cancer

作　　者：李坤[1] 侯宪芹[1] 胡波[2] 于晶功[2] 孙海娇[2] 周长江[1] 

机构地区：[1]大连大学附属新华医院消化内科,辽宁大连116021 [2]大连大学附属新华医院病理科,辽宁大连116021

出　　处：《中华肿瘤防治杂志》2011年第24期1948-1952,共5页Chinese Journal of Cancer Prevention and Treatment

摘　　要：目的:探讨直肠癌组织β-catenin和β-Trcp的表达及其临床意义。方法:应用免疫组织化学SP法检测直肠癌组织(60例)、直肠腺瘤组织(42例)和正常直肠黏膜组织(20例)中β-catenin和β-Trcp的表达情况。结果:直肠癌、直肠腺瘤和正常直肠黏膜组织中β-catenin的异位表达率分别为85.0%(51/60)、88.1%(37/42)和5.0%(1/20);膜缺失率分别为65.0%(39/60)、23.8%(10/42)和0(0/20);β-trcp的阳性表达率分别为63.3%(38/60)、57.1%(24/42)和0(0/20)。β-catenin的异位表达、膜表达缺失和β-Trcp的阳性表达与患者的年龄、性别、直肠癌的部位、形态、术前CEA水平、分化程度、Dukes分期和淋巴结转移及远处转移均无明显相关,P>0.05。β-Trcp阳性表达与β-catenin异位表达,在直肠癌组织中呈负相关,P<0.05。在直肠癌和直肠腺瘤组织中分别有18.3%(11/60)和35.7%(15/42)的β-catenin异位高表达同时伴有β-Trcp表达减弱或缺失,分别有58.3%(35/60)和21.4%(9/42)的β-cate-nin膜缺失同时伴有β-Trcp表达减弱或缺失。结论:β-catenin的异位表达和β-Trcp的阳性表达是直肠癌发生发展过程中的早期事件,两者可能成为直肠癌预防及早期诊断的重要标志。OBJECTIVE： To detect the expression of β-catenin and β-Trcp,and its clinical significance in the tissues of rectal cancer sequence.METHODS： The expression of β-catenin and β-Trcp were detected by the immunohistochemistry SP staining method in 60 rectal carcinomas tissues,42 rectal adenoma tissues and 20 normal rectal mucosal tissues.RESULTS： The ectopic expression rates of β-catenin in rectal carcinomas tussion,rectal adenoma tissues and normal rectal mucosal tissue were 85.0%（51/60）,88.1%（37/42）,5.0%（1/20）.The membrane fault rates of β-catenin in the three tissues were 65.0%（39/60）,23.8%（10/42） and 0（0/20）,respectively.The ectopic expression and membrane faulting expression of β-catenin,the positive expression of β-Trcp were not correlated with the age,gender,tumorous location,tumorous morphology,CEA,histology grade,Duke stage,lymph mode metastasis and remote metastasis（P0.05）.The positive expression of β-Trcp and the ectopic expression of β-catenin had a significant negative correlation in rectal carcinoma（P0.05）.There were 18.3%（11/60） and 35.7%（15/42） ectopic over expression of β-catenin,of which the β-Trcp was reduced or absente expression in rectal carcinomas tussion and rectal adenoma tissues respectively.Besides,there were 58.3%（35/60） and 21.4%（9/42） membrane fault expression of β-catenin,of which the β-Trcp was reduced or absente expression in rectal carcinomas tussion and rectal adenoma tissues respectively.CONCLUSION： The ectopic expression of β-catenin and the positive expression of β-Trcp are the early events in the rectal cancer sequence,and β-catenin and β-Trcp may be the tumor markers for prevention and early diagnosis of rectal cancer.

关 键 词：直肠肿瘤 免疫组织化学 WNT信号传导通路 Β-CATENIN β-Trcp 

分 类 号：R735.37[医药卫生—肿瘤]