白细胞介素23在大鼠脑梗死模型中的表达  

Experimental study on expression of IL-23 in rat cerebral infarction model

作　　者：吴乃君[1] 唐元海 金秀平[1] 田清伟[1] 魏剑芬[1] 王颖[1] 陈冬[1] 马绍杰[1] 贾伟[1] 

机构地区：[1]河北联合大学附属医院内分泌科,河北唐山063000 [2]河北省遵化市医院,064200

出　　处：《重庆医学》2012年第6期584-585,共2页Chongqing medicine

摘　　要：目的通过建立大鼠脑梗死模型,探讨白细胞介素-23(IL-23)在大鼠脑梗死区域炎性损伤中的表达。方法 (1)将健康成年雄性SD大鼠50只随机分为脑梗死组和假手术组,每组25只。脑梗死组按梗死后不同取材时间分为6、24、48、72h及7d 5个亚组,每组大鼠5只;假手术组按梗死组对应时间点分为6、24、48、72h及7d5个亚组,每组大鼠5只。(2)用线栓法制作左侧大鼠大脑中动脉阻塞(MCAO)模型。(3)采用HE、免疫组化染色,观察脑梗死区域光镜下形态学变化及IL-23的表达。(4)比较两组大鼠在同一时间点脑组织形态学变化及IL-23的表达。结果 (1)假手术组各时间点脑组织结构基本正常,无明显水肿。脑梗死组MCAO术后6h脑组织病变区域脑组织轻度水肿。24～48h水肿加重;(2)梗死组IL-23阳性细胞个数各时间点均高于假手术组,差异有统计学意义(P<0.01)。梗死组IL-23随时间的延续变化,在梗死后6h表达增高,并且在梗死后48h达到高峰,72h后阳性细胞数表达逐渐减少,各时间点IL-23比较差异有统计学意义(P<0.001)。结论 IL-23在大鼠脑梗死区域表达均显著增高,而且在24～48h达高峰。Objective To investigate the expresion of IL-23 on the inflammatory lesion in rat cerebral infarction area by establishing the rat cerebral infarction model.Methods（1）50 healthy male SD rats were randomly divided into 2 groups： infarction group（IS） and sham operation group（SH）.The IS group was further divided into 5 subgroups（n=5 each subgroup） according to the infarction duration of 6 h,24 h,48 h,72 h,7 d.The expression of IL-23 was measured by immunohistochemistry.（2）The rat model was established by using the occlusion method of left middle cerebral artery.（3）We studied the morphology changes after MCAO under light microscope,IL-23 expression by using HE staining,light microscope and immunohistochemistry.（4） Comparing changes of morphology,IL-23 expression of each group at the same time point.Results（1）The cellular structure was normal in sham operation group under light microscopy.There were swollen cells at 6 h after operation in the model group.There were large numbers of swollen cells after 24-48 h.（2） IL-23 positive cells were found after 6 h,reached peak after 24~48 h,and reduced after 72h.Compared with sham operation group,IL-23 positive cells expression was higher after 6h,24 h,48 h,3 d and 7 d,showing significant difference（P0.01,P0.001）.Conclusion The expression of IL-23 is increased in rat cerebral infarction area and reaches the peak at 24~48h.

关 键 词：白细胞介素23 脑缺血 炎症反应 

分 类 号：R743.3[医药卫生—神经病学与精神病学]