急性髓系白血病M2b型分子生物学特征的鉴定  

Characterization of Molecular Biological Features of Acute Myelogenous Leukemia M2b

作　　者：孟庆祥[1] 王建祥[1] 肖志坚[1] 郝玉书[1] 李建波[1] 卞寿庚[1] 刘汉芝[1] 钱林生[1] 王立[1] 薛艳萍[1] 

机构地区：[1]中国医学科学院中国协和医科大学血液学研究所血液病医院,天津市300020

出　　处：《中国肿瘤临床》2000年第3期165-169,共5页Chinese Journal of Clinical Oncology

基　　金：国家自然科学基金!( 3 93 70 3 19);天津市自然科学基金!( 9414 0 90 18);卫生部重点项目基金

摘　　要：目的 :研究急性髓系白血病 (AML) M2 b细胞遗传学和分子生物学特征 ,探讨 AML- M2 b与 t(8;2 1)白血病的关系。方法 :应用 G显带技术分析染色体核型 ,用 RT- PCR法检测 AML 1/ MTG8融合基因转录本并进行微量残留白血病检测 ,采用 Southern Blot技术检测 AML 1及 MTG8基因重排。结果 :86 .1% (31/ 36例 )的 AML -M2 b具有 t(8;2 1) ,13.9% (5 / 36例 )无 t(8;2 1)。在 AML- M2 b中 ,AML1/ MTG8融合基因转录本的检出率为10 0 % (44 / 44例 ) ,其中包括 5例无 t(8;2 1)的病例 ,AML1基因和 MTG8基因的重排检出率分别为 81.8%和71.0 %。 13例完全缓解 (CR)的 AML- M2 b中 12例可检出 AML1/ MTG8融合基因转录本。结论 :AML- M2 b与国际上的 t(8;2 1)白血病所指为同一疾病实体 ,AML 1/ ETO融合基因是这种疾病的基因标志 ,AML - M2 b可分为 t(8;2 1) (+) AML 1/ ETO(+) AML - M2 b和 t(8;2 1) (- ) AML 1/ ETO(+) AML - M2 b。经化疗获 CR的 AML - M2 b仍可检出微量残留白血病细胞。Objective: To investigate the cytogenetic features and molecular biological features of acute myelogenous leukemia M2b (AML M2b) and the relationship between AML M2b and t (8;21) leukemia. Methods: In patients with AML M2b, karyotype was studied by using G banding technique, AML1/MTG8 fusion gene transcript by using RT PCR and the rearrangement of AML1 gene and MTG8 gene by using Southern Blot technique.Results: The frequencies of AML M2b with or without t (8;21) were 86.1%(31/36) and 13.9%(5/36) respectively. AML1/MTG8 fusion gene transcript occured not only in all 44 AML M2b(44/44) including 5 cases without t (8;21), but also in 12 of 13 cases during complete remission. The rearrangement of AML1 gene and of MTG8 gene was found in 81.8% and 71.0% of AML M2b respectively.Conclusion: AML M2b and t (8;21) leukemia is one and the same disease entity in which AML1/MTG8 fusion gene is its molecular marker.AML M2b could be subclassified into t (8;21)(+)AML1/ETO(+)AML M2b and t(8;21)( ) AML1/ETO(+) AML M2b. AML1/MTG8 fusion gene transcript still may occur in AML M2b during complete remission.

关 键 词：急性髓系白血病 AML-M2b 细胞遗传学 分子生物学 

分 类 号：R733.71[医药卫生—肿瘤]