氢氧化钙调节骨代谢的体外研究  

Mechanisms of bone repairment in periapical diseases:studies on adjusting bone metabolism with calcium hydroxide in vitro

作　　者：梁宇红[1] 王嘉德[1] 于世凤[2] 庞淑珍[2] 

机构地区：[1]北京医科大学口腔医学院牙体牙髓科,100081 [2]北京医科大学口腔医学院病理科,100081

出　　处：《中华口腔医学杂志》2000年第2期112-114,共3页Chinese Journal of Stomatology

摘　　要：目的　观察氢氧化钙对骨组织成骨活性的影响 ,为根尖周病药物治疗学提供实验依据。方法　利用与牙槽骨生物学特点相似的小鼠颅骨建立体外培养模型 ,观察氢氧化钙作用下骨组织碱性磷酸酶 (alkalinephosphatase ,ALP)分泌活性及无机磷含量的变化。结果　适当浓度的氢氧化钙可以提高成骨细胞ALP的分泌活性 ,同时培养基中无机PO4 3 浓度也呈现有意义的变化。结论　Ca(OH) 2具有激活成骨细胞钙化酶活性、增加局部环境中PO4 3 浓度的作用 ,使矿物沉积更易于发生 ,促进根尖病变修复。Objective To provide experiment evidence for clinical practice and for further exploration on mechanism and medicinal control of periapical diseases Methods The culture model of neonatal mouse calvariae which has the similar biological activity to alveolar bone was established in vitro to observe the influence of calcium hydroxide on osteo activity Results The results showed that calcium hydroxide with proper concentration at organ culture level could enhance osteoblast ALP secreting activity, and increased the tendency of bone formation At the same time , the concentration of inorganic PO 4 3 in culture medium also had significant changes Conclusions The mechanism of calcium hydroxide promoting repairment of bone tissues is not only by providing rich Ca 2+ and alkaline environment mineral deposition, but also by stimulating calcification enzyme activity of osteoblast Meanwhile, increased PO 4 3 concentration might improve the mineralization environment, which causes mineralization easily

关 键 词：成骨细胞 碱性磷酸酶 氢氧化钙 根尖周围病 

分 类 号：R781.34[医药卫生—口腔医学]