干旱胁迫下马铃薯幼苗DNA甲基化研究  

DNA Methylation in Potato Under Drought Stress

作　　者：李媛媛[1] 程鹏[2] 熊兴耀[2] 洪亚辉[1] 

机构地区：[1]湖南农业大学生物科学技术学院,湖南长沙410128 [2]湖南农业大学湖南省马铃薯工程技术研究中心,湖南长沙410128

出　　处：《中国马铃薯》2012年第1期11-16,共6页Chinese Potato Journal

基　　金：国家科技支撑计划"高产优质专用马铃薯育种技术研究及新品种选育"(2006BAD01A06-1-8)

摘　　要：马铃薯(Solanum tuberosum L).是我国重要的粮食作物,性喜阴湿冷凉的环境,干旱严重影响着马铃薯的品质产量的稳定。试验采用PEG-6000模拟干旱胁迫处理3个品种的马铃薯幼苗,并对其基因组DNA进行甲基化敏感扩增多态性(Methylation-sensitive amplified polymorphism,MSAP)分析,在20%的PEG胁迫下,‘大西洋’的基因组DNA甲基化程度随着时间的增长而有所增大。‘中薯3号’在20%的PEG胁迫下,随着时间的增长,甲基化基本趋势是减弱的。‘中薯5号’和‘中薯3号’情况类似,其甲基化趋势基本上也是随着时间增长而降低的。将特异带回收测序和Blast比对,结果表明其中几条带跟与萜类化合物合成等功能基因具有一定的同源性。Potato is an important food crop, and adapts to cool and wet environments. However, drought seriously affects its quality and yield. In this research, three potato cultivars were treated with PEG-6000 to simulate drought stress. The extent and pattern of cytosine methylation of the genomes were assessed by using the technique of methylation-sensitive amplified polymorphism （MSAP）. It was found that treated with 20% PEG-6000, DNA methylation of＇Altantic＇ was increased, while that of DNA methylation of ＇Zhongshu 3＇ and ＇Zhongshu 5＇ decreased. Several variable MSAP profiles were isolated and sequenced, and the results were analyzed with Blast analysis. Blast analysis results showed that sequences had homology with some functional genes.

关 键 词：马铃薯 DNA甲基化 MSAP 

分 类 号：S532[农业科学—作物学]