STAT3在人肺腺癌细胞A549中的表达及意义  

Expression and significance of STAT3 in lung adenocarcinoma cells

作　　者：蓝秀[1] 王超[2] 辛彩霞[2] 宋本艳[2] 王鸿程[2] 吕祝庆[1] 吕伟玲[1] 孙德彬[1] 

机构地区：[1]丽水市中心医院呼吸内科,323000 [2]泸州医学院附属医院呼吸内科,646000

出　　处：《国际呼吸杂志》2012年第4期245-248,共4页International Journal of Respiration

摘　　要：目的探讨信号转导和转录激活因子3（STAT3）的临床应用价值。方法利用电化学发光免疫法检测人肺腺癌细胞A549和人胚胎成纤维细胞MRC-5培养3d的培养液中癌胚抗原（CEA）的表达水平。采用荧光定量PCR法检测人肺腺癌细胞A549和人胚胎成纤维细胞MRC-5中STAT3mRNA、CEAmRNA的表达。结果CEA在A549细胞培养液中的表达水平高于MRC-5细胞培养液[（31138±30．58）ng／LVS（3．10±3．70）ng／L，P〈0．05]；STAT3mRNA、CEAmRNA在A549细胞中的表达水平（6．96±3．37，5．68±2．70）均明显高于MRC-5细胞（1．01±0．19，1．02±0．21）（P〈0．01），且在A549细胞中STAT3和CEA的表达均呈正相关（r=0．521，P=0．047）。结论STAT3有望成为-种新的诊断肺腺癌的肿瘤标志物。Objective To investigate the clinical significance of signal transducer and activator of trascription 3 （STAT3）. Methods The eleetrochemiluminescence was used to detect the levels of carcinoembryonic antigen （CEA） in medium which cultured lung adenocarcinoma cell A549, human normal lung cell MRC-5 3 days. Relative expression of mRNA of STAT3,CEA in lung adenocarcinoma cell A549 and human normal lung cell MRC-5 was tested by real time PCR. Results The expression of CEA in lung adenocarcinoma cell A549＇s medium was higher than that in human normal lung cell MRC-5 E（31.38±30.58） ng/L vs （3.10±3.70） ng/L, P〈0. 051. The mRNA levels of STAT3,CEA in A549 cell （6.96 ±3.37,5.68±2.70 ） were apparently higher than those in MRC-5 cell （ 1.01 ±0.19,1.02± 0.21） （P〈0.01）. The mRNA levels of STAT3 had a positive correlation with CEA in lung adenocarcinoma cell A549 （ r =0. 521, P =0. 047）. Conclusions STAT3 can be served as a new marker of lung adenocareinoma.

关 键 词：肺腺癌 肿瘤标志物 信号转导和转录激活因子3 癌胚抗原 

分 类 号：R734.2[医药卫生—肿瘤]