非小细胞肺癌组织中生物钟基因Per2和血管内皮生长因子的表达及其临床意义  

Expression of circadian gene Per2 and VEGF in NSCL tissue and its clinical significance

作　　者：池闯[1] 林晓铭[1] 何志锋[1] 刘瑜[1] 孙成超[1] 

机构地区：[1]温州医学院附属第一医院胸心外科,浙江省温州325000

出　　处：《中国基层医药》2012年第4期491-493,共3页Chinese Journal of Primary Medicine and Pharmacy

基　　金：浙江省温州市科技局立项课题（Y20100039）

摘　　要：目的研究生物钟基因Per2（CircadiangenePer2）和血管内皮生长因子（VEGF）在非小细胞肺癌中的表达，探讨其临床意义。方法采用免疫组织化学染色法检测60例非小细胞肺癌组织和20例正常肺组织中Per2和VEGF的表达，分析其表达与临床病理特征的关系。结果非小细胞肺癌组织中Per2阳性表达率为71．7％，低于正常肺组织中的95．0％（x2=4．683，P〈0．05），而VEGF的阳性表达率为58．3％，高于正常肺组织中的15．0％（x2=11．295，P〈0．01）。非小细胞肺癌中Per2的表达缺失与分化程度及TMN分期相关（∥=4．413，6．179，均P〈0．05），而VEGF的阳性表达与分化程度及淋巴结转移相关（x2=6．524，P〈0．05）。两者在非小细胞肺癌中的表达呈负相关（r=-0．381，P〈0．01）。结论Per2在非小细胞肺癌中表达降低，而VEGF则呈高表达，两者在非小细胞肺癌的发生、发展中可能起相反的作用，影响其预后。Objective To detect the expression of Per2 and VEGF in NSCLC tissue, and analyze its clinical significance. Methods The expression of Per2 and VEGF was measured in 60 NSCLC and 20 normal lung tissue by immunohistochemistry assay, then the relation with clinicopathological parameters was analyzed. Results The positive expression rate of Per2 in NSCLC and in normal lung tissue were 71.7% and 95.0% （ X2 =4.683 ,P 〈0.05 ） ,while the positive expression rate of VEGF were 58.3% and 15% （ X2 = 11. 295, P 〈 0.01 ）. The negative expression of Per2 in NSCLC was correlated with pathological differentiation or TNM stage（ X2 = 4.413,6. 179 ,all P 〈 0.05 ） ,while the positive expression of VEGF in NSCLC was correlated with pathological differentiation or lymphatic metastasis （X2 =6.524,P 〈 0.05）. The expression of Per2 was negatively correlated with the expression of VEGF in NSCLC （r = -0.381 ,P 〈 0.01 ）. Conclusion The overexpression of VEGF in NSCLC may contribute to invasion and metastasis, while Per2 may be a beneficial prognosis factor in patients with NSCLC.

关 键 词：癌 非小细胞肺 生物钟基因Per2 血管内皮生长因子 免疫组织化学 

分 类 号：R734.2[医药卫生—肿瘤]