5种中药注射剂对多柔比星所致心肌H9c2损伤保护作用  

Protective Effect of Five Injections on Doxorubicin-Induced Cytotoxicity in Cardiac Myoblasts H9c2

作　　者：辛艳飞[1] 周国亮[1] 韩滨[1] 由振强[1] 宣尧仙[1] 

机构地区：[1]浙江省医学科学院安全性评价研究中心,浙江杭州310013

出　　处：《中华中医药学刊》2012年第3期483-485,共3页Chinese Archives of Traditional Chinese Medicine

基　　金：国家自然科学基金资助项目(30701064);国家"十二五"科技重大专项资助项目(2011ZX09301-003);浙江省科技计划项目(2010F10026;2009C13024);浙江省中医药管理局资助项目(2007CB187)

摘　　要：目的:考察5种中药注射剂对多柔比星(DOX)作用下H9c2心肌细胞损伤的保护作用。方法:应用H9c2心肌细胞筛查体系,通过检测细胞存活率、过氧化物岐化酶(SOD)活性和氧化代谢产物(MDA)含量,考察参麦注射剂、丹参注射剂、冠心宁注射剂、香丹注射剂和黄芪注射剂等5种中药注射剂对暴露于1.7×10-4至2.2×10-9M浓度DOX下H9c2心肌细胞的存活率的影响。结果:黄芪注射剂和香丹注射剂能提高DOX作用下心肌细胞的存活率,有效地缓解DOX所引起的心肌细胞MDA浓度增高,提高SOD酶活性且不影响DOX的体外抗肿瘤活性。结论:黄芪注射剂和香丹注射剂能降低DOX对心肌细胞的毒性作用,可能是通过增加抗氧化物酶活力,减少氧化产物生成,从而减轻氧化应激损伤这些途径实现的。Objective：To test whether five injections can transfer its antioxidant function into the protection of the doxorubicin （DOX） -induced cardiotoxicity in cardiac myoblasts H9c2. Method： H9c2 cells were exposed to DOX and/or five injection. The peak concentration of five injection of protective effect on DOX - induce was evaluated through exami- nation, level of malondialdehyde （MDA） and superoxide dismutase （SOl）） activity. The effects of five injections on the cytotoxicity of DOX to H9c2 cells and Asg9 lung carcinoma cells were studied. Results ： Huangqi injection and Xiangdan injection had protective effect on H9c2 cells against DOX and significantly increased SOD activity and decreased the MDA level in H9c2 experimental system damaged by DOX exposure while not attenuate the antitumor activity of DOX on A549 carcinoma cells. Conclusion：Our evidence indicates that Huangqi injection and Xiangdan injection elicited a typical protective effect on DOX -induced cytotoxicity in cardiac myoblasts H9c2 via suppressing oxidative stress.

关 键 词：多柔比星 H9C2心肌细胞 丹参注射剂 参麦注射剂 冠心宁注射剂 香丹注射剂 黄芪注射剂 

分 类 号：R285.5[医药卫生—中药学]