Fmr1基因敲除小鼠耳蜗GAD的表达  

Expression of GAD of cochlea in FMR1 gene Knock-out mice

作　　者：李敏雄[1] 杜娜[1] 孙卫文[1] 黄月玲[2] 沈岩松[1] 戴丽军[1] 陈盛强[1] 马钊恩[1] 张建国[1] 

机构地区：[1]广州医学院附属第二医院,广东广州510260 [2]广州医学院动物实验中心,广州510182

出　　处：《解剖学研究》2012年第1期21-25,F0004,共6页Anatomy Research

基　　金：广东省自然科学基金(815101700100005);广东省科技计划项目(2008B030301371;2009B030801368;2009B060300018);广东省中医药管理局建设中医药强省科研基金(2008184)

摘　　要：目的对4周龄Fmr1基因敲除小鼠的耳蜗的GAD表达进行观察,探讨耳蜗GAD的表达是否受FMRP的影响。方法使用PCR技术对Fmr1基因敲除小鼠鉴定后对4周龄的Fmr1基因敲除小鼠和野生型小鼠各15只进行耳蜗的苏木精-伊红(HE)染色和GAD免疫组织化学的表达观察,数据采用多因素方差分析处理。结果耳蜗HE染色结果:4周龄组KO鼠较WT鼠形态学观察无差异。4周龄KO小鼠的耳蜗中GAD表达的平均阳性细胞数均高于WT小鼠,P<0.01,差异具有统计学意义。结论 GAD表达的改变可能与FMR1基因KO小鼠听源性惊厥发病有关。Objective Observe 4 weeks FMR1 KO mice and WT mice cochlea morphology and expression of GAD of cochlea.Methods 4 weeks Fmr1 knockout mice were identified using the PCR technica,and using the way of immunohistochemistry to compare with the changes of GAD,expression between FMR1 KO mice and WT mice cochlea.Results There were no difference between FMR1 KO mice and WT mice cochlea morphology by HE dyeing.Increased expression of GAD in cochlear in FMR-1KO mice（P 0.01）.Conclusion Increased expression of GAD in cochlear in 4 weeks FMR-1KO mice is related with audiogenic seizure susceptibility of Fmr1 knockout mice.

关 键 词：听源性惊厥 脆性X综合症 Fmr1敲除小鼠 谷氨酸脱羧酶 

分 类 号：R749.94[医药卫生—神经病学与精神病学]