β-连环蛋白在结肠癌细胞及肿瘤球细胞中的表达  

Expression of β-catenin protein in human colon cancer cells and their tumor sphere cells

作　　者：时伟红[1] 王惟[1] 杨葳[1] 郭晓冲[1] 于萌[1] 魏政立[1] 秦英[1] 郑志红[1,2] 

机构地区：[1]中国医科大学实验动物部,辽宁沈阳110001 [2]中国医科大学病理与病理生理学研究室,辽宁沈阳110001

出　　处：《现代肿瘤医学》2012年第3期486-489,共4页Journal of Modern Oncology

基　　金：辽宁省科学技术项目(编号:2009408001-3);辽宁省科学技术项目(编号:2009408001-1)

摘　　要：目的:研究信号通路β-连环蛋白在人结肠癌ccl187细胞及其肿瘤球细胞中的表达状况。方法:培养ccl187细胞及其肿瘤球,分别提取ccl187细胞和肿瘤球细胞的总RNA,应用半定量RT-PCR和Westernblot方法分别检测β-连环蛋白在ccl187细胞及其肿瘤球中的mRNA水平及蛋白水平的表达。结果:半定量RT-PCR结果显示ccl187细胞和肿瘤球细胞中β-连环蛋白转录水平分别为41.6%和42.6%(P>0.05)。半定量Western blot结果显示ccl187细胞和肿瘤球细胞中β-连环蛋白的含量分别为36.7%和73.4%(P<0.05)。结论:信号通路β-连环蛋白在人结肠癌ccl187细胞及其肿瘤球细胞中转录水平基本一致,但肿瘤球细胞中β-连环蛋白被积累,含量显著高于ccl187细胞。Objective：To study the expression of signaling pathway β-catenin in human colon cancer ccl187cells and their tumor sphere cells.Methods：ccl187 cells and their tumor sphere cells were cultured.RNA was extracted respectively from ccl187 cells and their tumor sphere cells.The mRNA of β-catenin was examined by Semi-quantitive RT-PCR.The protein of β-catenin was examined by western blot.Results：Semi-quantitive RT-PCR results shown that the transcription level of ccl187 cells and their tumor sphere cells were 41.6% and 42.6% respectively（P0.05）.Western blot results showed that the translation level of ccl187 cells and their tumor sphere cells were 36.7% and 73.4% respectively（P0.05）.Conclusion：The transcription level of β-catenin of ccl187 cell and tumor sphere cells is comformable,but theβ-catenin protein is accumulated in tumor sphere cells.The amount of β-catenin protein in tumor sphere cells is higher than that of ccl187 cell markedly.

关 键 词：ccl187细胞系 Β-连环蛋白 肿瘤球细胞 结肠癌 

分 类 号：R735.35[医药卫生—肿瘤]