左旋氨氯地平对成年大鼠神经干细胞氧／糖剥夺损伤的保护作用  

Levamlodipine protects neural stem cells from oxygen/glucose deprivation injury in adult rats

作　　者：郭阳[1] 张培东 谭盛[1] 陈健[1] 陈瑞清[1] 徐如祥[3,4] 陈镇洲 汤浩[1] 

机构地区：[1]广东省脑功能修复与再生重点实验室,南方医科大学珠江医院神经内科,广州510282 [2]心血管内科 [3]神经外科 [4]北京军区总医院八一脑科医院神经外科,北京100700

出　　处：《中华神经医学杂志》2012年第3期222-227,共6页Chinese Journal of Neuromedicine

基　　金：国家自然科学基金（30801184）;广东省药学会基金（2011X19）;广东省科技计划重点专项（2011A0304000071

摘　　要：目的研究左旋氨氯地平对成年大鼠神经干细胞氧／糖剥夺（OGD）损伤后细胞生长活力、增殖和凋亡情况的影响．探讨左旋氨氯地平对OGD后成年大鼠神经干细胞的保护作用。方法制备成年大鼠海马神经干细胞0GD模型，按干预的左旋氨氯地平浓度分为4组：0μmol／L组（即无干预对照组）、0．5μmol／L组、1．0μmol／L组、5．0μmol／L组。CCK-8法检测不同浓度左旋氨氯地平对0GD后成年大鼠神经干细胞生长活力的影响：Edu荧光染色法观察不同浓度左旋氨氯地平对0GD后成年大鼠神经干细胞增殖的影响：AnnexinV．FITC／PI双染流式细胞仪检测0GD后成年大鼠神经干细胞的凋亡情况。结果OGD6h后成功制备成年大鼠海马神经干细胞0GD模型。与无干预对照组比较，不同浓度左旋氨氯地平干预后成年大鼠海马神经干细胞的生长活力均明显增加，差异有统计学意义（P〈0．05）；5．0μmol／L左旋氨氯地平组OGD后神经干细胞增殖率明显增加，差异有统计学意义（P〈0．05）；1．0μmol／L和5．0μmol／L左旋氨氯地平组0GD后神经干细胞中凋亡细胞比例明显减少，差异有统计学意义俨〈0．05）。结论成年大鼠神经干细胞OGD模型中，左旋氨氯地平具有一定的抗0GD损伤的保护作用。Objective To explore the effects of Levamlodipine on viability, proliferation and apoptosis of neural stem cells after hypoxia-ischemia injury in adult rats. Methods Hypoxic-ischemic damage to adult neural stem cells was simulated in the established oxygen/glucose deprivation （OGD） models. Four groups were designed according to Levamlodipine concentrations： 0μmol/L, 0.5 μmol/L, 1.0 μmol/L and 5.0 μmol/L. Effects of Levamlodipine at 4 different concentrations on the viability, proliferation and apoptosis ofhippocampal neural stem cells after OGD were tested by CCK-8 assay, Edu fluorescence staining and flow cytometry （Annexin V-FITC/PI）, respectively. Results Models of hypoxia-ischemia damage to hippocampus neural stem cells were successfully established by OGD for 6 hours. Compared with control group （0 μmol/L）, the viability of hippocampal neural stem cells was significantly increased in the other 3 groups （0.5μmol/L, 1.0 μmol/L and 5.0 μmol/L） （P〈0.05）. The proportion of proliferating cells after OGD was significantly increased at S phase in 5.0 μmol/L Levamlodipine group （P〈0.05）. The proportion of apoptotic cells after OGD was significantly decreasedin 1.0 μmol/L and 5.01μmol/L Levamlodipine groups （P〈0.05）. Conclusion Levamlodipine may protect neural stem cells from hypoxic-ischemic injury in adult rats.

关 键 词：左旋氨氯地平 神经干细胞 氧/糖剥夺 

分 类 号：R743.3[医药卫生—神经病学与精神病学]