E1B缺陷性腺病毒对人恶性脑胶质瘤的溶瘤作用  

The oncolytic effect of E1B mutant adenovirus on human malignant gliomas

作　　者：李良平[1] 俞方毅[1] 陈家祥[1] 许智蕾[1] 吴少瑜[2] 王文雅[2] 吕琳[2] 饶进军[2] 

机构地区：[1]广州市红十字会医院神经外科,广州510220 [2]南方医科大学药学院临床药理学科组,广州510515

出　　处：《中华神经医学杂志》2012年第3期235-237,共3页Chinese Journal of Neuromedicine

摘　　要：目的研究E1B缺陷性腺病毒d11520对人恶性脑胶质瘤细胞株U251的体外溶瘤作用。方法按感染复数（MOI）为50、5、0．5、0．05、0．005、0pfu将E1B缺陷性腺病毒d11520感染常规培养的U251、Hep3B、T24细胞（Hep3B为阳性对照，T24为阴性对照1，结晶紫染色观察细胞病变（CPE）出现的时间；MOI为5pfu时病毒空斑试验检测d11520在3种细胞中的复制。将携带报告基因βgal的腺病毒载体Ad．βgal感染细胞，计算d11520对3种细胞的感染率。结果结晶紫染色结果显示Hep3B细胞对d11520最敏感、发生CPE效应最快，U251次之，而T24则不产生CPE效应；d11520在U251细胞中复制数和感染率均低于Hep3B细胞，但明显高于T24细胞，差异均有统计学意义俨〈0．05）。结论E1B缺陷性腺病毒d11520对人恶性脑胶质瘤细胞具有显著的溶瘤作用。Objective To investigate the oncolytic effect of E1B mutant adenovirus （d11520） on human malignant gliomas. Methods Ad-βgal vector was used to investigate the infectibility of d11520. U251, Hep3B （positive control） and T24 （negative control） cell lines were infected with d11520 respectively at 50, 5, 0.5, 0.005 and 0 pfu of multiplicity of infection （MOI）. The replication efficiency of d11520 in host cells was assessed by plaque assay. The cytopathic effect （CPE） was assessed by crystal violet staining in a panel of tumor cells. Results Crystal violet staining showed the Hep3B cell line was the most sensitive to dl1520 and had the fastest CPE, followed by the U251 cell line, while the T24 cell line had no CEP. The replication and infection rates ofdl1520 in the U251 cell line were lower than in the Hep3B cell line but significantly higher than in the T24 cell line （P〈0.05）. Conclusion The E1B mutant adenovirus （d11520） has a significant oncolytic effect on human malignant gliomas.

关 键 词：神经胶质瘤 E1B缺陷性腺病毒 细胞病变效应 

分 类 号：R73[医药卫生—肿瘤]