透明质酸软骨链蛋白探针对肺肿瘤小鼠放疗靶区勾画的可行性分析  

Feasibility of cartilage link protein of hyaluronic acid for defining radiotherapeutic target volume in a mouse model of lung tumor

作　　者：梁智欣[1] 强永刚[1] 廖永华[1] 

机构地区：[1]广州医学院实验核医学教研室,广东广州510182

出　　处：《南方医科大学学报》2012年第3期301-305,共5页Journal of Southern Medical University

基　　金：国家自然科学基金(81071152);广州科技计划项目(2008J1-C261);广州市教育局项目(61049)~~

摘　　要：目的提取牛鼻HA软骨链蛋白(HA-CLP),确定其对化学致癌小鼠模型肿瘤边界的指示情况,为肺肿瘤影像学感兴趣区(ROI)边界的勾画提供实验依据。方法建立化学致癌小鼠肺肿瘤模型,用亲和层析法从牛鼻软骨分离纯化HA-CLP,后以125I标记HA-CLP行放射性自显影显像研究,并对肺肿瘤行免疫组织化学分析及蛋白免疫印迹分析。结果实验收获HA-CLP(241.8±63.4)mg(n=5),抽提蛋白率为2.42%;肺肿瘤放射性自显影在2 h显像最清晰;免疫组织化学在2 h能最清晰区分肿瘤边界,其HA-CLP免疫信号最强;肿瘤HA-CLP蛋白免疫印迹均在2 h条带显示最清晰。结论自提物HA-CLP具HABP家族免疫原性,通过应用自提物HA-CLP放射自显影实验以及组织免疫组织化学提示HA-CLP对肺肿瘤边界有良好的指示作用。Objective To investigate the feasibility of using cartilage link protein of hyaluronic acid（HA-CLP） for defining the tumor boundary in a mouse model of lung carcinoma.Methods Lung carcinoma was induced in KM mice by chemical carcinogenesis.HA-CLP separated from bovine cartilage and purified by affinity chromatography was labeled with 125I for autoradiography.Immunohistochemical analysis and Western blotting were used to examine the efficiency of HA-CLP in defining the boundaries of the lung tumors.Results With autoradiography,the clearest image of lung cancer was obtained at 2 h.With immunohistochemical method,the tumor boundary was the most clearly displayed at 2 h when the strongest signals of HA-CLP was detected;Western blotting also showed the clearest bands of HA-CLP at 2 h.Conclusion HA-CLP has the immunogenicity of HABP,and can efficiently indicate lung tumor boundary in autoradiography and immunohistochemistry.

关 键 词：透明质酸软骨链蛋白 肿瘤模型 肿瘤边界 放疗靶区 肺肿瘤 

分 类 号：R734.2[医药卫生—肿瘤]