PKC-a、K-ras蛋白在结直肠癌组织中的表达及意义  

Expression and significance of PKC-a and K-Ras proteins in colorectal cancer

作　　者：宋芳[1] 万义增[2] 陈素贤[2] 

机构地区：[1]辽宁医学院研究生学院,辽宁锦州121000 [2]辽宁医学院附属第三医院

出　　处：《山东医药》2012年第7期29-31,共3页Shandong Medical Journal

摘　　要：目的探讨蛋白激酶C(PKC)及K-ras蛋白在结直肠癌发生、发展中的作用。方法采用免疫组化法分别检测76份结直肠腺癌组织(腺癌组)、15份结直肠腺瘤组织(腺瘤组)及10份正常结直肠黏膜(正常组)中PKC-a及K-ras蛋白表达,分析两者的相关性及其与肿瘤临床病理特征的关系。另采用Western blot法检测30份结直肠癌黏膜组织和10份癌旁黏膜组织(距肿瘤10 cm)中PKC-a和K-ras蛋白表达水平。结果腺癌组PKC-a阳性率明显低于、K-ras阳性率明显高于腺瘤组及正常组,P均<0.05。PKC-a和K-ras表达与结直肠癌临床病理分级有关,随着病理分级增加,PKC-a表达率逐渐降低,K-ras升高;二者表达与性别、年龄、淋巴结转移及Dukes分期均无关。PKC-a与K-ras蛋白的表达之间呈负相关(r=-0.930)。结论 PKC-a蛋白与K-ras蛋白与结直肠癌的发生及发展有关。Objective To investigate the roles of protein kinase C（PKC） and K-ras protein in the occurrence and development of colorectal cancer.Methods Immunohistochemistry method was used to detect the PKC-a and K-ras protein expression in 76 colorectal adenocarcinoma（adenocarcinoma group）,15 colorectal adenoma tissue（adenoma group） and 10 normal colorectal mucosa（normal group）,their relationship and correlation with clinical pathological features of the tumor were analyzed.Western blot method was used to detect the PKC-a and K-ras protein expression level in 30 colorectal cancer tissues and 10 adjacent mucosa（away from the tumor 10 cm）.Results The positive rate of PKC-a and K-ras in adenocarcinoma group was 35.5% and 68.4%,the former was significantly lower than that in normal mucosa,adenoma,while the later was on the opposite（all P0.05）.PKC-a and K-ras expression in colorectal carcinoma were correlated with clinical pathologic grading,with the increase of pathological grading,PKC-a expression rate reduced gradually,K-ras expression elevated;expression of the two indexes were not correlated with gender,age,lymph node metastasis and Dukes stage（P0.05）.PKC-a and K-ras protein expression was negatively correlated（r=-0.930）.Conclusion PKC-a protein and K-ras protein are correlated with the development and progression of colorectal cancer.

关 键 词：结直肠癌 蛋白激酶C K-RAS 免疫组化法 WESTERN BLOT 

分 类 号：R365[医药卫生—病理学]