生物酶法提取沙棘果渣总黄酮工艺的优化  

Optimization of Bio-enzyme Method for Total Flavones Extraction from Marc of Sea Buckthorn

作　　者：徐升运[1] 赵文娟[1] 陈卫锋[2] 任平[1] 秦涛[2] 

机构地区：[1]陕西省科学院酶工程研究所,西安710600 [2]陕西省酶工程技术中心,西安710600

出　　处：《湖北农业科学》2012年第5期983-986,共4页Hubei Agricultural Sciences

基　　金：中国科学院西部之光项目(2007KDF-05)

摘　　要：用生物酶法提取沙棘(Hippophae rhamnoides)果渣中的总黄酮,通过单因素试验和正交试验优化提取工艺。结果表明,酶解体系各因素对总黄酮提取率影响的大小顺序为酶用量、酶解温度、酶解时间、pH,最佳提取条件为生物酶用量占沙棘果渣质量的4%、pH 5、酶解温度60℃、酶解时间120 min,料水质量比1∶30,此条件下总黄酮提取率可达到0.796%。与有机溶剂回流法和超声波法相比,生物酶法所得沙棘果渣的总黄酮提取率分别提高了51.91%和48.51%。Bio-enzyme method was applied for total flavones extraction from marc of sea buckthorn （Hippophae rhamnoides）; and the extraction craft was optimized by single factor experiments and orthogonal design. The results showed that the opti- mum extraction conditions for the enzyme reaction system were as follows, enzyme dose, 4% of the mass of marc of sea buckthom; pH, 5; Temperature, 60 ℃; Time, 120 min; Mass ratio of material to water, 1：30. Under the optimum condi- tions, the yield of total flavones was up to 0.796%. Compared with heat reflux extraction and ultra-sonic extraction, the yield of bio-enzyme extraction was increased by 51.91% and 48.51%, respectively.

关 键 词：生物酶法 沙棘(Hippophae rhamnoides)果渣 总黄酮 提取工艺 

分 类 号：R284.2[医药卫生—中药学] S793.6[医药卫生—中医学]