CISH、IHC联合检测胃腺癌EGFR、HER2基因状态及其临床意义  

Combined detection of EGFR and HER2 in gastric adenocarcinoma by CISH and IHC

作　　者：朱献斐[1] 赵亚娟[1] 刘海芳[1] 黄震[1] 

机构地区：[1]义乌市中心医院,浙江省义乌322000

出　　处：《现代实用医学》2012年第1期26-28,F0003,共4页Modern Practical Medicine

基　　金：浙江省义乌市科技局资助项目(2008-3-07)

摘　　要：目的比较显色原位杂交(CISH)与免疫组织化学(IHC)检测胃腺癌表皮生长因子(EGFR)及表皮生长因子受体2(HER2)的基因状态。方法 CISH及IHC检测85例胃腺癌组织EGFR、HER2基因扩增及蛋白表达。结果 EGFR蛋白表达阳性29例,其中表达(3+)的4例中,4例基因扩增;表达(2+)的11例中,8例基因扩增;表达(1+)的14例中,1例基因扩增。HER2蛋白表达阳性13例,其中表达(3+)的4例中,4例基因扩增;表达(2+)的6例中,5例基因扩增;表达(1+)的3例中,无基因扩增。EGFR、HER2蛋白表达及基因扩增无相关性。结论 IHC检测蛋白表达仅(3+)与CISH检测基因扩增有较好一致性,CISH检测基因状态有较好特异性。Objective To compare the expressions of epidermal growth factor（EGFR） and human epidermal growth factor receptor 2（HER2） in gastric adenocarcinoma by chromogenic in situ hybridization（CISH） and immunohisto- chemistry （IHC）, respectively. Methods EGFR and HER2 expressions of 85 cases with gastric adenocarcinomas were detected by CISH and IHC. Results Among 29 patients with positive EGFR, four cases with （3＋） showed gene amplification; eight of 1 lcases with （2＋） showed gene amplification; only one of the 14 cases with （1＋） showed gene amplification. Among 13 patients with positive HER2, four cases with （3＋） showed gene amplification; five of six cases with （2＋） showed gene amplification; three cases with （1＋） failed to show gene amplification. There was no correlation between gene amplification and EGFR or HER2. Conclusions Cases with （3＋） oflHC is consistency with CISH preferably, and CISH has a high specificity in predicting gene status.

关 键 词：显色原位杂交 免疫组织化学 胃腺肿瘤 

分 类 号：R735.2[医药卫生—肿瘤]