13种哺乳动物OTR密码子使用偏性及其聚类分析  

Analysis of OTR Codon Bias and Cluster in 13 Mammal Species

作　　者：张之一[1] 陈蕾[1] 薛慧良[1] 徐金会[1] 徐来祥[1] 

机构地区：[1]曲阜师范大学生命科学学院

出　　处：《北华大学学报（自然科学版）》2012年第1期44-48,共5页Journal of Beihua University（Natural Science）

基　　金：国家重点基础研究发展计划项目(2007CB109104)

摘　　要：目的研究13种哺乳动物催产素受体基因（OTR）密码子使用偏性，并探讨其影响因素；通过对偏性情况进行聚类分析，研究其与系统发育的关系．方法计算RSCU，CBI，ENC等密码子偏性指标，与可能的几种影响因素做相关性分析，采用SPASS15．0软件，以相对同义密码子使用度为变量对偏性情况进行聚类分析．结果与结论1）CUG，GUG，GCC，UUC，AUC，CGC为哺乳动物OTR使用频率较高的密码子；2）密码子第3位碱基的GC含量是影响哺乳动物OTR密码子偏性的主要因素，基因的GC含量次之，而芳香族氨基酸的含量和蛋白疏水水平对其密码子使用偏性的影响不大；3）对于功能相同的基因，亲缘关系相近的物种密码子使用偏性指标较接近，但某些物种仍有一定差异．Objective To study the codon bias of oxytocin receptor （OTR） gene in 13 mammal species, to explore its impacting factors and the phylogenetic relationships by clustering the codon bias. Methods Bias indexes of RSCU, CBI and ENC were calculated and the relevant analysis of several potential impacting factors was performed. SPASS 15.0 software was used for the clustering analysis of the codon bias, in which the relative synonymous eodon usage was taken as the variable. Results and Conclusion 1 ） CUG, GUG, GCC, UUC, AUC, CGC were found to be the frequently used codon;2）The number of GC located on the third of codon is the main factor that affects the eodon bias of OTR gene in mammalians, the number of GC in genes is the secondary factor, and aromatic amino acid content and hydrophobic protein level have little effect on the codon bias;3 ） For the genes with the same functions, the codon bias indexes are similar among species with close genetic relationship except for some special species.

关 键 词：哺乳动物 OTR 密码子偏性 聚类分析 

分 类 号：Q95[生物学—动物学]