电离辐射诱发胎鼠的形态及胎脑组织甲基化结合蛋白MeCP2改变  

The Study on Morphologic Alteration of Fetal Mice and the Change of MeCP2 in Fetal Brain Induced by Ionizing Radiation

作　　者：陈凤[1] 张凤翔[1] 涂彧[2] 

机构地区：[1]鄂尔多斯市中心医院,内蒙古鄂尔多斯017000 [2]苏州大学放射医学与防护学院,江苏苏州215123

出　　处：《中国辐射卫生》2012年第1期13-15,共3页Chinese Journal of Radiological Health

基　　金：长江学者和创新团队发展计划资助(IRT0849)

摘　　要：目的探讨电离辐射诱发体内旁效应损伤表现及可能机制。方法将孕9d昆明小鼠随机分为7组:空白对照组、0.5Gy全身照射组、0.5Gy头部照射组、1.0Gy全身照射组、1.0Gy头部照射组、2.0Gy全身照射组及2.0Gy头部照射组,照射组用60Co治疗机对实验小鼠于孕9d时进行单次急性垂直照射,于孕18d取胎鼠,观察胎鼠状态,记录胎鼠个数、大小,有无死胎、畸胎及流产等,取活胎快速断脑取脑组织。用western-blot方法检测胎鼠脑组织中MeCP2蛋白含量变化。结果与空白对照组比较,电离辐射照射孕鼠后,随着剂量的增加,胎鼠死胎率、畸胎率、流产率呈上升趋势;除0.5Gy全身照射组外,其余各剂量组MeCP2表达量明显降低,组间无明显差异。结论电离辐射作用于孕鼠后,能够诱发胎脑旁效应,无论是直接照射腹部还是作用头部,在一定剂量范围内,随着剂量的增加可能导致确定性效应和随机性效应增加,如死胎、畸胎、发育障碍发生率增加。Objective In order to investigate the effect and the possible mechanism of γ- rays on neurodevelopment of fetal brain tissue as bystander effect organ. Methods pregnant kunming mice were randomly divided into blank control group, 0.5 Gy whole - body exposed group, 0.5 Gy head exposed group, 1.0 Gy whole - body exposed group, 1.0 Gy head exposed group, 2.0 Gy whole - body exposed group and 2.0 Gy head exposed group. The exposed mice were exposed with a vertical single acute dose using 60Co therapy apparatus on the 9 th day of pregnancy, and cesarean operation were performed to gain fetal mice on the 18th day of pregnancy. The number, the size, stillbirth, birth defects and abortion, and get fetal brains from live births were observed. Western - blot assay was used to detect the expression of MeCP2 protein. Results Compared with the blank control group, the rates of stillbirth, birth defects and abortion ascended as the increase of doses ; the expression of MeCP2 were upregulated except 0.5 Gy whole - body exposed group, there were no significant differences between groups. Condusion When the pregnant mice were exposed to ionizing radiation in the first trimester, bystander effect in fetal brain tissue was induced, within a certain range, the incidence of deterministic effects and stochastic effects ascended as the increase of doses.

关 键 词：电离辐射 胎脑 MECP2 体内旁效应 

分 类 号：Q691.5[生物学—生物物理学]