发育期补充鱼油对大鼠脑内脂肪酸组成及神经递质的影响  

Effect of fish oil supplementation on fatty acid composition and neurotransmitters of growing rats

作　　者：李红娟[1] 刘德华[1] 张恩平[2] 

机构地区：[1]山西医科大学儿少卫生教研室,太原030001 [2]山西医科大学公共卫生学院分析中心

出　　处：《卫生研究》2000年第1期47-49,共3页Journal of Hygiene Research

基　　金：山西省自然科学基金!(No.951032)

摘　　要：为观察发育期补充二十二碳六烯酸(DHA)对大鼠脑内脂肪酸代谢和部分神经递质的变化,将孕鼠分为4组:正常对照组(O)、添加DHA 的低(L)、中(M)、高(H)剂量组,于基础饲料中分别添加鱼油0、17.5、35和70m l/kg,实验组饲料中的DHA含量分别为7.5、15和30g/kg。以幼鼠脑匀浆中脂肪酸分析、大脑皮质和海马内单胺类神经递质5-羟色胺(5-HT)、去甲肾上腺素(NE)、多巴胺(DA)及肽类神经递质生长抑素(SS)作为检测脑神经生化代谢的指标,并监测孕鼠、仔鼠体重。结果表明:补充DHA的各剂量组幼鼠脑匀浆中DHA含量及海马中5-HT、DA、SS含量均显著高于对照组,但补充DHA各组仔鼠体重增长不同程度地受到抑制。结论:1.发育期补充DHA使大鼠脑内脂肪酸代谢发生变化,进而调节递质释放;2.对人和动物补充DHA的同时,应考虑EPA(C20v5n-3)、AA(C20v4 n-6 )In order to observe the change of brain fatty acid composition and neurotransmitter by DHA supplementation during the development stage of young rats,pregnant rats were randomly divided into four groups:normal control group(O),low(L),medium(M) and high(H) dose DHA supplemented groups.The fatty acids composition,the levels of 5 hydroxy tryptamine(5 HT),dopamine(DA),norepinephrine(NE) and somatostatin(SS)in ceretral cortex and hippocampus of offsprings were measured.The body weight of pregnant rats and offsprings were also observed. The results showed that DHA content of brain homogenate and the levels of 5 HT,DA and SS in hippocampus of experimental young rats were significantly higher than those of the control group.And the body weight of young rats in the three DHA supplemented groups were retarded in different degree.It was concluded that DHA supplementation changed the fatty acid composition and increased the levels of 5 HT,DA and SS in hippocampus.Proper ratio of n 3 and n 6 fatty acids in dietary supplementation should be considered.

关 键 词：二十二碳六烯酸 脂肪酸 神经递质 

分 类 号：Q593.2[生物学—生物化学] O623.617[理学—有机化学]