间接法原位PCR检测喉鳞癌组织HPV感染  

Detection of HPV infection in larynx squamous cell carcinoma tissues by indirect in situ polymerase chain reaction

作　　者：李晓[1] 吴能定[1] 徐如君[1] 沈宝珠[1] 黎向红[1] 

机构地区：[1]杭州市第一人民医院病理科,浙江杭州310006

出　　处：《细胞与分子免疫学杂志》2000年第1期45-48,共4页Chinese Journal of Cellular and Molecular Immunology

摘　　要：目的建立稳定的原位PCR方法 ,并探讨HPV感染与喉鳞癌发生的关系。方法采用免疫组化、原位杂交、PCR和间接原位PCR技术 ,检测了50例喉鳞癌中的HPV感染情况。结果免疫组化衣壳抗原阳性者6例(12 % ) ,原位杂交阳性者13例 (26 % ) ,PCR阳性者10例 (20 % ) ,原位PCR阳性者17例 (34 % ) ,综合上述方法的检出率为42 % (21例 )。结论HPV感染与喉癌有着明显的关系 ,间接原位PCR在检测HPV感染中具有较高的敏感性、特异性和可靠性 ,可以定性、定位。Aim To make up a stable method of in situ PCR and to probe the relationship between HPV infection and larynx squamous cell carcinomas. Metheds HPV infection in 50 specimens of larynx squamous cell carcinoma was examed by immunohistochemical staining. in situ hybridization,PCR and indirect in situ PCR. Results The positive detection rate of HPV DNA was 12%by immunohistochemistry, 26%by in situ hybridization,20%by PCR, 34%by in situ PCR, and totally 42%in twenty one out of fifty cases. Conclusion HPV infection is related to the occurrence of larynx squamous cell carcinomas. Indirect in situ PCR has better sensitivity,reliability, and specificity than other methods. It is reliable and has the role of diagnosis of localization and nature of the lesion.

关 键 词：乳头瘤病毒 喉鳞癌 免疫组化染色 喉肿瘤 PCR 

分 类 号：R739.650.4[医药卫生—肿瘤]