加温诱导肿瘤细胞凋亡与逆转多药耐药性的实验研究  

Research on inducement of apoptosis and revrsion of multidrug resistance in human tumor cell line KBV200 by hyperthermia

作　　者：徐建业[1] 周琦[2] 卢萍[1] 汤伟 董莲芳 

机构地区：[1]重庆市肿瘤研究所中心实验室,400030 [2]重庆市肿瘤研究所妇瘤科,400030 [3]重庆市肿瘤研究所药房,400030

出　　处：《中华理疗杂志》2000年第1期33-36,共4页Chinese Journal of Physical Therapy

基　　金：国家教委及国家人事部留学回国人员科研基金!( 教外司留[1995]135 号)

摘　　要：目的　探讨加温逆转肿瘤细胞株ＫＢＶ２００ 多药耐药性及与诱导细胞凋亡的关系。方法用四唑蓝快速比色法（ＭＴＴ法） 检测细胞存活率；用形态学（ 光镜、电镜观察） ；生化学（ＤＮＡ 末端转移标记法，ＴＵＮＥＬ法） 及流式细胞术分析细胞凋亡状况。结果　加温４２ ℃，１ ｈ 可明显降低ＫＢＶ２００ 细胞存活率，提高长春新碱细胞毒性作用；单加ＶＣＲ 细胞存活率为（９０ ．７０ ±８．７８） ％ ，加ＶＣＲ 加温为（５７ ．４０±２１ ．５５）％ ，Ｐ＜ ０ ．００２。加温与细胞分化剂ＣＤＡ－ Ⅱ合用，有明显协同逆转作用。ＣＤＡ－ Ⅱ与ＶＣＲ合用细胞存活率为（５９ ．２４ ±３０．８０）％ ，加温与ＣＤＡ－ Ⅱ及ＶＣＲ 合用为（１５ ．３２ ±２ ．６８）％ ，Ｐ＜ ０．０５。４２ ℃或４３ ℃处理１，２４ ｈ 后即可观察到明显细胞凋亡。４３ ℃１ ｈ 处理诱导作用更强。结论　加温可明显逆转肿瘤细胞多药耐药及诱导细胞凋亡。加温对肿瘤细胞凋亡的诱导，在加温逆转肿瘤细胞耐药性中可能起着重要作用。Objective To investigate the relationship between the reversing multidrug resistance and inducing apoptosis in human tumor cell line KBV200 by hyperthermia.Methods Cell surviving fraction(SF) was determined by MTT assay.Apoptosis in KBV200 was detected by light and electron microscope,DNA end-labeling method(Tunel) and flow cytometry.Results SF of KBV200 was decreased and cytotoxicity of Vincristine(VCR) on KBV200 was increased by hyperthermia 42℃ for 1 hour.The SF of KBV200 with single VCR treatment was (90.70±8.78)%;in combination of hyperthermia with VCR,the SF was down to (57.40±21.55)%.There was a significant difference(P<0.002) in two groups.During the combining use of hyperthermia and cell differentiation agent(CDA-II),the reversal effect on KBV200 was obviously increased;with CDA-II and VCR treatment,the SF of KBV200 was (59.24± 30.80)%; when the combination of hyperthermia,VCR and CDA-II together,the SF of (15.32± 2.68)% was received(P<0.05).Apoptosis occurred after 24 hours of hyperthermia with 42℃ or 43℃ for 1 hour .Inducement of apoptosis was stronger with 43℃ than it with 42℃.Conclusion Hyperthermia could reverse the multidrug resistance and induce the apoptosis in KBV200.Apoptosis of KBV200 induced by hyperthermia is an important factor in reversal multidrug resistance on KBV200.

关 键 词：高温 抗药性 肿瘤 细胞凋亡 实验研究 热疗法 

分 类 号：R73－37[医药卫生—肿瘤] R454.5[医药卫生—临床医学]