白介素-18在急性胰腺炎大鼠中性粒细胞凋亡中的作用  

Role of IL-18 in peripheral neutrophil apoptosis in rats with sever acute pancreatitis

作　　者：吴彦彦[1] 许兰涛[2] 

机构地区：[1]苏州大学研究生部,江苏省苏州市215021 [2]上海交通大学附属第六人民医院奉贤分院消化科,上海市201400

出　　处：《世界华人消化杂志》2012年第2期140-144,共5页World Chinese Journal of Digestology

基　　金：上海市卫生局基金资助项目;No.2009226~~

摘　　要：目的:探讨白介素-18(IL-18)在重症急性胰腺炎(SAP)时外周血中性粒细胞(PMN)凋亡中的作用.方法:SD大鼠60只,随机分为2组,每组30只,分别建立大鼠急性坏死性胰腺炎组(ANP)及假手术组(SO)模型,在制模后3、6、12h分期分批处死大鼠,抽取下腔静脉血测定血淀粉酶含量及血清中IL-18的含量,作中性粒细胞分离并检测外周血中性粒细胞凋亡率;同时对大鼠胰腺组织行病理切片HE染色,并对胰腺组织进行病理评分.结果:ANP组中性粒细胞(PMN)凋亡率较SO组明显降低,并随着时间的延长逐渐明显(P<0.01),至术后12h降至2.15%±0.45%;ANP组血清中IL-18的含量较SO组明显升高,而且随着时间的延长更明显(P<0.01),在12h达到4705.70±296.30;ANP组PMN的凋亡率与血清中IL-18的水平呈负相关(r=-0.78,P<0.01).结论:ANP组外周血PMN的凋亡明显延迟,血清中IL-18的含量明显增高,IL-18可能在急性胰腺炎外周血的PMN凋亡中发挥作用.AIM：To examine the role of interleukin-18 （IL18） in apoptosis of peripheral blood neutrophils （PMNs） in rats with severe acute pancreatitis （SAP）.METHODS：Sixty SD rats were randomly divided into two groups：SAP group and sham operation （SO） group.At 3,6 and 12 h after SAP induction,blood samples were collected from the inferior vena cava to measure serum amylase and IL 18;PMNs were separated to detect their apoptosis;and pancreatic specimens were taken for pathological examination.RESULTS：PMN apoptosis rate decreased remarkably in the SAP group than in the SO group,and the decrease became more obvious over time （P0.01）.Serum levels of IL-18 in the SAP group increased significantly compared with those in the SO group （P0.01）.There was a negative correlation between the apoptosis rate of PMNs and serum levels of IL-18 in SAP rats （r=-0.742,P0.01）.CONCLUSION：Compared to control rats,PMN apoptosis was obviously delayed and serum levels of IL-18 remarkably increased in SAP rats,indicating that IL-18 may play an important role in peripheral neutrophil apoptosis in SAP.

关 键 词：白介素-18 急性胰腺炎 中性粒细胞 凋亡 

分 类 号：R576[医药卫生—消化系统]