高效液相色谱测定不同生境黄芩中的黄芩苷  

Measurement of Content of Baicalin from Scutellaria baicalensis in Different Habitat by HPLC

作　　者：龙月红[1] 邢朝斌[1] 劳凤云[2] 何闪[1] 梁能松[1] 李宝财[1] 

机构地区：[1]河北联合大学生命科学学院,河北唐山063000 [2]河北联合大学药学院,河北唐山063000

出　　处：《中国实验方剂学杂志》2012年第6期97-100,共4页Chinese Journal of Experimental Traditional Medical Formulae

摘　　要：目的:测定不同生境条件下,黄芩根、茎和叶中黄芩苷的含量。方法:采用HPLC,Kromasil ODS柱(4.6 mm×250mm,5μm),以甲醇-水(53:47)为流动相,检测波长280 nm,线性范围0.124～0.93μg,r=0.999 9,平均回收率98.17%,RSD1.3%(n=6)。结果:不同生境下黄芩根的黄芩苷平均含量分别为6.79%,6.01%,5.49%,3.50%,4.38%、茎的黄芩苷平均含量分别为0.10%,0.08%,0.09%,0.06%,0.03%;叶的黄芩苷平均含量分别为0.27%,0.26%,0.15%,0.14%,0.09%。结论:本方法简便快速,稳定可靠,重现性好。相同产地不同生境中的黄芩及黄芩不同器官的黄芩苷含量间存在显著差异。Objective： To measure the content of baicalin in root, stem and leave of Scutellaria baicalensis growing in different habitat. Method： The content of baicalin was determined by HPLC. The chromatographic condition included Kromasil ODS （4.6 mm × 250 mm, 5μm） and mobile phase consisting of a mixture of methanol-H2O （53：47）. Baicalin was detected at 280 nm. The standard curve was linear in the range of 0. 124-0.93 μg with correlation coefficient 0. 999 9. The average recovery of baicalin was 98.17% with RSD 1.3% （n =6）. Result： The average content of baicalin in different habitat root was 6.97% , 6.01% , 5.49% , 3.50% and 4.38% respectively. In different habitat stem, the average content of baicalin was 0. 10% , 0.08% , 0. 09% , 0.06% and 0. 03% respectively. In different habitat leave, the average content of baicalin was 0. 27% , 0.26% , 0. 15% , 0. 14% and 0. 09% respectively. Conclusion： The method of distill is simple and stable. The determination method was quick with good reappearance. Produced in the same area, the content of baicalin of S. baicalensis in different habitat and different organ had significant different.

关 键 词：黄芩 生境 高效液相 黄芩苷 含量 

分 类 号：R284.1[医药卫生—中药学]