siRNA干扰Pokemon基因影响A549细胞增殖的实验研究  

Empirical study on the anti-proliferation effect of siRNA against pokemon on human lung cancer cell line A549

作　　者：谢勇[1] 江涛[1] 

机构地区：[1]重庆医科大学附属第一医院呼吸内科,重庆400016

出　　处：《中国现代医学杂志》2012年第5期42-46,共5页China Journal of Modern Medicine

摘　　要：目的研究siRNA干扰Pokemon基因对肺腺癌A549细胞增殖抑制效应的变化。方法专业设计合成3条针对Pokemon的siRNA,分别转染A549细胞后,RT-PCR检测转录水平Pokemon mRNA表达的变化,筛选出其中最高效的1条siRNA;用MTT法检测该siRNA干扰Pokemon对A549细胞增殖的抑制作用;流式细胞技术检测该siRNA干扰Pokemon对A549细胞凋亡的影响。结果 3条siRNA均成功转染A549细胞,倒置荧光显微镜下观察细胞呈圆绿色。RT-PCR结果显示有2条siRNA使细胞中Pokemon的mRNA表达降低(P<0.05)。MTT法结果显示siRNA干扰Pokemon后对A549细胞增殖有抑制作用(P<0.05),其中48 h抑制效率达(24.14±1.39)%。流式细胞技术检测结果显示该siRNA干扰Pokemon可增加A549细胞的凋亡,凋亡率为14.05%。结论应用RNA干扰Pokemon基因能够抑制A549细胞的增殖,促进A549细胞的凋亡。Pokemon基因有可能成为肺癌治疗中的一个新靶点。【Objective】 To investigate the anti-proliferation effect of siRNA against pokemon on human lung cancer cell line A549.【Methods】 We professionally devised and synthesized three siRNAs against pokemon,then transfected it into A549 cells.We detected the mRNA expression of transcription level changes by RT-PCR experiment and screened out the most efficient siRNA.The effects on cell proliferation and apoptosis were analyzed by MTT assay and flow cytometry.【Results】 The three siRNAs were successfully transfected into A549 cells and the cells were green under fluorescence microscope cylindrical.The experiment of RT-PCR showed that the expression of pokemon mRNA in two siRNA groups were lower than the control groups.The MTT assay showed that the anti-proliferation effect of siRNA on A549 cells were significant（P 0.05）,the anti-proliferation rate at 48 h were（24.14±1.39）%.Flow cytometry revealed that the siRNA on the A549 cells increased the apoptotic rate,the rate was 14.05%.【Conclusions】 Application of RNA interference against pokemon gene on human lung cancer cell line A549 can inhibit the proliferation and promote apoptosis.Pokemon gene in lung cancer treatment may become a new target.

关 键 词：POKEMON SIRNA A549 细胞增殖 

分 类 号：R734.2[医药卫生—肿瘤]