BMP-2、bFGF和Dex联合应用对犬牙牙周膜细胞成骨分化能力的影响  

The effect of combination use of BMP-2、bFGF and Dex on osteogenic differentiation of periodontal ligament cells in dogs

作　　者：宋扬[1] 金作林[1] 宋镜明[1] 赵祝[1] 杨雪静[1] 

机构地区：[1]第四军医大学口腔医学院正畸科,陕西西安710032

出　　处：《临床口腔医学杂志》2012年第3期135-137,共3页Journal of Clinical Stomatology

基　　金：国家自然科学基金资助(30973358)

摘　　要：目的:观察骨形成蛋白(BMP-2)、碱性成纤维细胞生长因子(bFGF)和地塞米松(Dex)联合应用对犬牙周膜细胞(PDLCs)成骨分化能力的影响。方法:将第4代犬PDLCs分为5组:bFGF+Dex、BMP-2+bFGF、BMP-2+Dex、BMP-2+bFGF+Dex、对照组。检测各组碱性磷酸酶(ALP)活性,并应用RT-PCR检测各组PDLCs成骨关键基因OPN、COL-1的表达。结果:第7、14 d,各组ALP表达均较空白组显著增强(P<0.05),其中200μg/L BMP-2+10μg/LbFGF+10-8mol/L Dex诱导组犬PDLCs的ALP活性显著高于其他各组(P<0.05)。RT-PCR显示,200μg/L BMP-2+10μg/L bFGF+10-8mol/L Dex诱导组OPN、COL-1基因表达最为显著。结论:3种诱导因子两两结合均能增强犬PDLCs成骨能力,但在最佳浓度下3种因子联合应用诱导能力最强(P<0.05)。Objective： To investigate the effect of combined application of BMP-2,bFGF and Dex on osteogenic differentiation of periodontal ligament cells （PDLCs） in dogs. Method：The fourth generation of PDLCs were randomly divided into 5 groups： bFGF＋Dex group,BMP-2＋bFGF group, BMP-2＋Dex group, BMP-2＋bFGF＋Dex group and the control group. Osteogenic differentiation of PDLCs was evaluated by quantitative alkaline phosphatase （ALP） assay and the RT-PCR analysis for the expression of the key osteogenic gene including OPN,COL-1. Result：After 7, 14 days,ALP activities increased significantly in all 4 groups （P 〈0.05） than the control. The osteogenic differentiation of PDLCs in the group with BMP-2＋bFGF＋Dex were enhanced much more significantly than other groups （P 〈0.05）. Conclusion：h suggested that combined application of 200 μg / L BMP-2, 10 μg / L bFGF and 10^-8mol / L Dex can remarkably promote the osteogenic differentiation of PDLCs.

关 键 词：牙周膜细胞 骨形成蛋白-2 碱性成纤维细胞生长因子 地塞米松 

分 类 号：R781.4[医药卫生—口腔医学] Q813.1[医药卫生—临床医学]