Jab1和p27^(kip1)基因在喉鳞状细胞癌组织和喉癌Hep-2细胞中的表达及意义  

Expression and their significance of Jab1 p27^(kip1) in laryngeal squamous cell carcinoma and Hep-2 cells

作　　者：李发萍[1] 曹华[2] 吴玉瑛[2] 

机构地区：[1]广东省人民医院(广东省医学科学院)耳鼻咽喉科,广州510080 [2]郑州大学第一附属医院耳鼻咽喉科

出　　处：《临床耳鼻咽喉头颈外科杂志》2012年第6期252-255,259,共5页Journal of Clinical Otorhinolaryngology Head And Neck Surgery

摘　　要：目的:探讨Jab1和p27kip1基因在喉鳞状细胞癌组织和喉癌Hep-2细胞中的表达及意义。方法:采用免疫组织化学方法检测50例喉鳞状细胞癌组织和10例癌旁正常组织中Jab1和p27kip1的表达。采用人工合成Jab1siRNAⅡ用脂质体2000包裹后转染Hep-2细胞,用RT-PCR方法分析转染后喉癌细胞中Jab1和p27kip1基因的表达情况。结果:Jab1和p27kip1蛋白的阳性表达定位为细胞核中,Jab1在人喉鳞状细胞癌组织中高表达,正常喉黏膜中不表达或弱表达;p27kip1在人喉癌组织中弱表达,正常喉黏膜中强表达;两者呈负相关。Jab1siRNAⅡ可降低Jab1mRNA表达,并且随着时间的延长,Jab1mRNAⅡ在Hep-2细胞中的表达逐渐减弱,而p27kip1 mRNA的表达则无明显变化。结论:Jab1在人喉鳞状细胞癌组织中表达增强,正常喉黏膜中不表达或弱表达;p27kip1则正好相反。两者在喉癌中的表达呈负相关。Jab1siRNAⅡ可以特异性降低Jab1mRNA在喉癌Hep-2细胞中的表达,为进一步研究Jab1基因在喉癌中的作用奠定了基础。Objective：To discuss the expression and the significance of Jab1 p27kip1 in laryngeal squamous cell carcinoma and Hep-2 cells.Method：Immunohistochemical method was used to examine the expressions of Jab1 and p27kip1 proteins in 50 cases laryngeal squamous cell carcinomaes and 10 cases normal laryngeal tissues adjacent to laryngeal squamous cell carcinoma.Hep-2 cells were transfected with synthetic Jab1 siRNA by Lipofectamrne2000.RT-PCR method was adopted to examine the mRNA expression of the Jab1 and p27kip1 gene in Hep-2 cells which was treated with Jab1 siRNAⅡ.Result：Clearly brown staining restricted to nucleus was considered as positive expression of Jab1 and p27kip1 protein.The expression rate of Jab1 protein in laryngeal squamous cell carcinoma was significantly higher than that of normal laryngeal mucosa,and the expression rate of protein p27kip1in laryngeal squamous cell carcinoma was significantly lower than that of normal laryngeal mucosa.There was a negative relationship between Jab1 and p27kip1 protein in laryngeal squamous cell carcinoma.The expression of Jab1 mRNA was suppressed markedly after transfected by Jab1 siRNAⅡ.As the reaction time increased,the expression of Jab1 mRNA of Hep-2 cells decresed significantly,and the expression of p27kip1 mRNA remained unchanged.Conclusion：The expression rate of Jab1 protein in laryngeal squamous cell carcinoma is significantly higher than that of normal laryngeal mucosa.There is a negative relationship between Jab1 and p27kip1 protein in laryngeal squamous cell carcinoma.After transfected by Jab1 siRNAⅡin the Hep-2 cells,the expression of Jab1 mRNA is suppressed markedly.Jab1 siRNA would be a good methdology for the further study.

关 键 词：喉鳞状细胞癌 Jab1基因 P27KIP1基因 RNA干扰 HEP-2细胞 RT-PCR 

分 类 号：R739.6[医药卫生—肿瘤]