ω-3多不饱和脂肪酸对人胆囊癌细胞GBC-SD增殖的影响及机制的研究  

Effects of ω-3 polyunsaturated fatty acids on proliferation of human gallbladder carcinoma cell GBC-SD and mechanisms

作　　者：史继荣[1] 张翔宇[1] 朱静[1] 乔岐禄[1] 张宝善[1] 

机构地区：[1]北京大学第一医院普通外科,北京100034

出　　处：《中国现代医学杂志》2012年第6期14-18,共5页China Journal of Modern Medicine

摘　　要：目的研究二十二碳六烯酸（docosahexaenoicacid，DHA）和花生四烯酸（arachidonicacid，从）对人胆囊癌细胞GBC-SD增殖的影响，通过相关蛋白质指标，探讨可能的分子机制。方法MTT法检测不同浓度（10-80ug／mL）DHA和AA对胆囊癌细胞GBC-SD存活率的影响；免疫印迹检测β-catenin、c-myc、cychnD1三种蛋白表达量的变化。结果当DHA浓度达到60ug／mL时可明显抑制胆囊癌细胞GBC-SD存活并随着浓度升高抑制作用增强，呈剂量依赖（P〈o．05），而相同浓度AA无明显作用。同时β-catenin、c-myc、cychnD1三种蛋白表达量均下降。结论DHA可抑制人胆囊癌细胞GBC-SD增殖，使β-catenin、c-myc、cyclinD1表达减少。[ Objective ] To investigate the effect of DHA and AA on human carcinoma of gallbladder cell line cells GBC-SD in vitro and explore possible molecular mechanisms through the relevant protein targets. [ Methods ] GBC-SD cell viability is examined for different concentration （10-80 ug/M1） of DHA and AA by MTI assay. Protein expression of β-catenin, c-myc, cyclin D1 was measured by Western-blot. [Results] The DHA showed a dose de- pendent inhibition to the survival of GBC-SD after the concentration above 60 ug/mL（P 〈0.05）. However, there is no significant effect for AA treatment group. The expression of protein β-catenin, c-myc, cyclin D1 are all decreased by western-blot. [ Conclusion ] DHA can effectively inhabit the proliferation of human carcinoma of gallbladder cell line cells GBC-SD, decrease the expression of protein β-catenin, c-myc, cyclin D1.

关 键 词：Ω-3多不饱和脂肪酸 胆囊癌 WNT/Β-CATENIN通路 

分 类 号：R-33[医药卫生] R735.8