肝癌微波消融并间质治疗后外周血T淋巴细胞的变化和意义  

Changes of T lymphocyte cell subsets after microwave ablation combined with interstitial therapy in paitents with liver cancer

作　　者：全毅[1] 何柱光[1] 李祥厦[1] 

机构地区：[1]广东省肇庆市第一人民医院肿瘤科,广东肇庆526000

出　　处：《中国现代医学杂志》2012年第6期44-47,共4页China Journal of Modern Medicine

基　　金：广东省科技厅基金资助项目(No:2011B031800294)

摘　　要：目的观察肝癌间质治疗基础上联合微波消融治疗对患者外周血T淋巴细胞亚群的影响。方法收集我院40例原发性肝癌患者,其中单纯微波消融治疗组(A组)20例,微波消融联合间质治疗组(B组)20例,分别于消融治疗前、治疗后一周应用流式细胞仪检测两组患者外周血CD3+、CD4+、CD8+、CD4+/CD8+比值。结果治疗前两组患者外周血T淋巴细胞亚群相比较差异无显著性(P>0.05);A、B组手术后一周外周血CD3、CD4及CD4/CD8均较术前升高,手术前后相比较有显著性差异(P<0.05);A、B两组术后间比较,B组CD3、CD4及CD4/CD8值高于A组,CD8略低于B组,仅CD4有显著性差异(P<0.05)。结论微波消融治疗原发性肝癌可使外周血T淋巴细胞亚群增加,增强细胞免疫功能,而微波消融联合间质治疗后,可使免疫功能进一步得到显著改善。[ Objective ] To study the changes of T lymphocyte cell subsets after microwave ablation combined with interstitial therapy in patients with liver cancer before and after operation, [Methods] 40 patients with liver cancer were randomly divided into A group, 20 received microwave ablation alone, and B gro~!p, 20 received mi- crowave ablation combined with interstitial therapy of percutaneous lipiodol and anti-cancer agents ~t）jevt^on intratu- morly into liver cancer. Then T lymphocyte cell subsets in the peripheral blood, CD3~, CD4＊, CDS＊, CDg＊/CD8~, were measured by flow cytometry before and one week after operation. [ Results ] No difference in changes of T lympho- cyte cell subsets between A and B group preoperative （P 〉0.05）; It was higher of CD3, CD4, CD4/8 in postoperative than in preoperative of both A and B groups, there was significant difference between preoperative and postoperative in both A and B groups （P 〈0.05）. It was higher of CD3, CD4, CD4/8 of B group than of A group in postoperative, but there was significant difference only in CD4 between A and B group postoperativt~ ~P ~0,0~）~ ~ Conclusion ] Mi- crowave ablation combined with interstitial therapy for liver cancer may activate T lymphocyte cell sgbsets and im-~ prove immune function to much more extent than microwave ablation alone.

关 键 词：肝癌 TACE 消融 间质治疗 T淋巴细胞亚群 流式细胞仪 

分 类 号：R735.34[医药卫生—肿瘤]