HPLC-MS/MS法检测人血浆中游离花生四烯酸浓度  

HPLC-MS/MS determination of arachidonic acid in human plasma

作　　者：詹同运[1] 石磊[1] 赵树进[1] 曾晓晖[1] 贺宝霞[1] 关慧[1] 

机构地区：[1]广州军区广州总医院药剂科,广州510010

出　　处：《药物分析杂志》2012年第3期388-391,382,共5页Chinese Journal of Pharmaceutical Analysis

基　　金：广东省科技计划项目(2009B080701105)

摘　　要：目的:建立测定人血浆中花生四烯酸浓度的液相色谱串联质谱法。方法:以奥美拉唑为内标,待测血浆经乙酸乙酯萃取后,采用Agilent SB-C18(2.1 mm×100 mm,3.5μm)色谱柱,以水-甲醇(10∶90,v/v)为流动相,流速0.2 mL.min-1,柱温25℃;采用电喷雾离子源(ESI)负离子模式将样品离子化,多反应监测(MRM)模式下对花生四烯酸(m/z 303.2→259.2)和奥美拉唑(m/z 344.2→191.1)进行测定。结果:花生四烯酸在497.88～4997.24 ng.mL-1范围内线性关系良好(r=0.9989)。低、中、高浓度的平均回收率均大于70%,批内与批间RSD均小于10%。结论:本方法简单、快速、灵敏,适用于大样本量血浆中游离花生四烯酸的分析研究。Objective：To develop an HPLC-MS/MS method for determination of arachidonic acid in human plasma.Methods：Omeprazole was used as internal standard.Plasma was extracted with ethyl acetate and samples were separated on an Agilent SB-C18 column（2.1 mm×100 mm,3.5 μm）with a mobile phase of water-methanol（10∶ 90,v/v）.The flow rate was 0.2 mL·min-1 and the temperature of the column was 25 ℃.The eletrospray ionization（ESI）with negative source of the mass spectrometer and detected in multiple reaction monitor（MRM）mode to detect arachidonic acid（m/z 303.2→259.2）and omeprazole（m/z 344.2→191.1）.Results：The calibration curve was linear in the range of 497.88-4997.24 ng·mL-1（r=0.9989）for arachidonic acid.The recovery of the extraction of arachidonic acid was more than 70%;RSDs of the intra-assay and inter-assay were less than 10%.Conclusion：The method is simple,rapid,sensitive and suitable for determination of arachidonic acid in human plasma.

关 键 词：高效液相色谱串联质谱法 花生四烯酸 多不饱和脂肪酸 生理活性 生物样品 血药浓度 内源性物质 医学研究 临床诊断 

分 类 号：R917[医药卫生—药物分析学]