次声对骨髓间充质干细胞生长情况的影响  

The effect of infrasound on the growth of bone marrow mescenchymal stem cells

作　　者：毕迎立[1] 陈银海[2] 范建中[1] 

机构地区：[1]南方医科大学南方医院康复医学科,广州510515 [2]南方医科大学珠江医院门诊部

出　　处：《中华物理医学与康复杂志》2012年第3期166-170,共5页Chinese Journal of Physical Medicine and Rehabilitation

基　　金：基金项目：广东省自然科学基金项目（8451051501000460）;广州市海珠区科技计划项目（2009-Y-018）

摘　　要：目的观察次声对骨髓间充质干细胞（BMSCs）生长情况的影响，探讨次声合理应用的时间参数。方法采用全骨髓培养法获取SD大鼠的原代BMSCs，经传代纯化后，取P3代细胞分为次声作用组和空气暴露组。次声作用组分为次声作用10min组、30min组和60min组；空气暴露组分为空气暴露10min组、30min组和60min组。采用CCK8法检测细胞的增殖活性，流式细胞术进行细胞凋亡和细胞周期分析。结果经次声作用10min、30min和60min的BMSCs，在培养72h后，光密度（OD）值分别为（1．480±0．030）、（1．348±0．030）、（1．493±0．030），培养96h后，OD值分别为（1．774±0．030）、（1．731±0．030）、（1．833±0．030）；而空气暴露10min、30min和60min的BMSCs，在培养72h后，OD值分别为（1．479±0．030）、（1．267±0．030）、（1．227±0．030），培养96h后，OD值分别为（1．567±0．030）、（1．563±0．030）、（1．632±0．030）。经次声作用的BMSCs培养72h后，其OD值均大于空气暴露的BMSCs，且随着作用或暴露时间的增加，细胞OD值呈递增或先减后增的趋势，以作用或暴露时间为60min的BMSCs的OD值最高，2组间差异有统计学意义（P〈0．05）。次声作用10rain和60min时，BMSCs的早期凋亡率分别为（1．07±0．12）％和（0．97±0．21）％，相应暴露时间的空气暴露组的早期凋亡率分别为（1．43±0．06）％和（3．33±0．15）％，差异有统计学意义（P〈0．01）。60min的次声作用还可降低BMSCs的中晚期凋亡率、提高其正常细胞的百分率（P〈0．01）；次声作用30min时，对BMSCs的细胞凋亡没有显著影响。次声作用10min与次声作用30min相比，可对BMSCs的细胞周期产生相反的影响；次声作用10min组的DNA合成前期的（G1期）细胞多于空气暴露10min组（P〈0．05）；次声作用30min组G1期的细胞少于空气暴露30min组（P〈0．01），而次声作�Objective To investigate the effect of infrasound on the growth of bone marrow mescenchymal stem cells （BMSCs） and seek the most reasonable duration for using infrasound. Methods The primary BMSCs were obtained from Sprague-Dawley rats through whole bone marrow adherent cultivation. The cells of passage 3 were divided into trial groups treated with infrasound for 10 min, 30 min or 60 min, and control groups which were not treated with infrasound but exposed to air for the same durations. The vitality of cell proliferation was measured using the CCK8 method. Apoptosis and the cell cycle were analysed with flow cytometry （FACS）. Results After cultivation for 72 h, the optical density （OD） values for BMSCs treated with infrasound for 10 min, 30 min and 60 min were 1. 480 ± 0. 030, 1. 348 ± 0. 030,1. 493 ± 0. 030 respectively and after 96 h they were 1. 774 ± 0. 030, 1. 731 ± 0. 030 and 1. 833 ± 0. 030. All of which were significantly greater than in the control groups （ 1. 479 ± 0. 030, 1. 267 ± 0.030and 1.227±0.030after72 hand 1.567±0.030, 1.563±0.030 and 1.632±0.030 after 96 h）. With the extension of the treatment duration, the OD values of the BMSCs increased or increased after decreasing, and the OD value for BMSCs treated with infrasound for 60 min was the highest. The FACS results indicated no effect of infrasound on apoptosis of BMSCs when the treatment duration was 30 min, but that cell apoptosis could be inhibited when the treatment duration was 10 min or 60 min. The early phase apoptosis rates were 1.07% ± 0.12% and 0.97% ± 0.21% in the trial groups treated for 10 min and 60 min respectively, and 1.43 % ± 0.06% and 3.33% ± 0.15% in the respective control groups, a highly significant difference. The results of cell cycle analysis showed infrasound could disturb the cell division of BMSCs significantly when the treatment duration was less than 30 min, but there was no significant effect when the treatment duration was 60 rain. Conclusions Infrasound can promote cell proliferat

关 键 词：次声 骨髓 间充质干细胞 增殖 凋亡 细胞周期 

分 类 号：R363[医药卫生—病理学]