miR-126下调MMP-2抑制人脑胶质瘤细胞侵袭  

miR-126 Inhibits Invasion of Human Brain Glioma Cells by Decreasing MMP-2

作　　者：陈寿仁[1,2] 王占祥[2] 沈上杭[2] 谭国伟[2] 刘希尧[2] 朱宏伟[2] 

机构地区：[1]福建医科大学研究生教育学院,福州350004 [2]厦门大学附属第一医院神经外科

出　　处：《肿瘤防治研究》2012年第3期264-266,共3页Cancer Research on Prevention and Treatment

基　　金：福建省自然科学基金面上项目资助课题(2009D002);厦门市科技局基金资助项目(3502220077055)

摘　　要：目的初步探讨miR-126抑制人胶质瘤细胞侵袭的可能机制。方法化学合成miR-126,脂质体转染人脑胶质瘤U87细胞,应用RT-PCR、Western blot检测MMP-2基因和蛋白的表达情况,并应用Transwell小室检测转染前后细胞侵袭力的变化。结果 miR-126上调后U87细胞的MMP-2基因和蛋白表达降低,并且细胞侵袭力明显降低。结论化学合成的miR-126在抑制人胶质瘤细胞侵袭过程中发挥重要作用,可能成为胶质瘤基因治疗的新靶点。Objective To explore the mechanism underlying the inhibiting effects of miR-126 on the invasion of human brain glioma cells.Methods miR-126 was synthesized and transfected into the U87 malignant glioma cells by liposome.The relative expression of MMP-2 and MMP-9 gene was quantified by RT-PCR,the expression of MMP-2 and MMP-9 protein was detected by Western blot,and cell invasion was assessed by Transwell chamber assay.Results miR-126 inhibited the activity of MMP-2 and the invasion of human U87 glioma cells.Conclusion The chemically synthesized miR-126 plays an important role in cell invasion of human brain glioma cells,which may be a promising target for glioma gene therapy.

关 键 词：MIR-126 胶质瘤 基质金属蛋白酶-2 侵袭 

分 类 号：R739.41[医药卫生—肿瘤]