microRNA-31在原发性肺癌组织中的表达及临床意义  

Expression and Clinical Significance of microRNA-31 in Primary Lung Cancer Tumor Tissues

作　　者：王生[1,2,3] 王启鸣[2,3] 王慧娟[1,2,3] 李鹏[2,3] 马智勇[2,3] 樊青霞[1] 

机构地区：[1]郑州大学第一附属医院肿瘤科,郑州450052 [2]郑州大学附属肿瘤医院内科 [3]河南省肿瘤医院内科

出　　处：《肿瘤防治研究》2012年第3期281-284,共4页Cancer Research on Prevention and Treatment

基　　金：2011年河南省基础与前沿技术研究计划资助项目(112300410033);河南省卫生科技创新型人才工程专项经费资助项目(201052);河南省医学学术技术带头人出国培训项目

摘　　要：目的检测肺癌组织及癌旁正常组织中miR-31的表达情况与肺癌的诊断、肿块大小、淋巴结转移、吸烟及分期的关系。方法采用quantitative real-time RT-PCR方法对75例肺癌患者术后癌组织及癌旁正常组织中miR-31的表达量进行检测。结果 miR-31在癌组织中的相对表达量是癌旁正常组织的6.48倍(P<0.001)。吸烟患者miR-31的表达量是不吸烟患者的3.06倍(P=0.033)。在NSCLC中,与肿瘤长径≤3 cm肿瘤相比,长径>3 cm肿瘤中miR-31的表达量较高(P=0.045)。无论淋巴结转移与否,其表达量差异无统计学意义(P=0.703)。结论 miR-31在肺癌组织中高表达、在不同肿块中表达的差异以及与吸烟的关系,提示其可能成为肺癌的筛查、诊断及预测肿块大小的一个生物标志物。Objective Detected the miR-31 expresssion level in tumor tissues and adjacent normal tissues to observe the relationship between miR-31 and the screening diagnosis of lung cancer.Methods Using quantitative real-time RT-PCR to assess the miR-31 expression in lung cancer tumor tissues and adjacent normal tissues.Results The relative expression level of miR-31 is 6.48 folds in tumor tissues compared to adjacent normal tissues（P0.001）.The level in smoke patients specimen is 3.06 folds to non-smoke.The expression level of tumor major axis3 cm is higher than major axis≤3 cm in NSCLC（P=0.033）.Whether the lymph nodes metastasis or not,there is no difference between them.Conclusion The outcomes that miR-31 was overexpressed in lung cancer,and the difference in accordance with tumor major axis as well as the relationship with cigarettes indicated miR-31 might be a biomarker for screening,diagnosis,and predicting the tumor axis.

关 键 词：肺癌 MIRNA miR-31 诊断 生物标志物 

分 类 号：R734.2[医药卫生—肿瘤]