乙酰肝素酶和血管内皮生长因子C mRNA的表达与肺癌侵袭和转移的分子生物学  

Study of HPSE and VEGF-C mRNA expressions with invasiveness and metastasis in human lung cancer

作　　者：苏树伟[1,2] 田辉[1,2] 李林[1,2] 岳韦名[1,2] 高存[1,2] 司立博[1,2] 

机构地区：[1]山东省交通医院胸外科,山东济南250031 [2]山东大学齐鲁医院胸外科

出　　处：《中国老年学杂志》2012年第6期1114-1116,共3页Chinese Journal of Gerontology

基　　金：国家自然科学基金项目(No.30571844);山东省科技攻关项目(No.2009GG10002007);山东省自然科学基金项目(No.ZR2009CM090)

摘　　要：目的研究乙酰肝素酶(HPSE)和血管内皮生长因子(VEGF)-C mRNA的表达是否与肺癌的侵袭性生长和淋巴结转移有关。方法应用RT-PCR技术检测HPSE和VEGF-C mRNA在65例肺癌组织中的表达,并与癌旁组织和正常肺组织对比,同时结合肺癌的临床病理学特征及预后进行分析。结果肺癌组织中HPSE和VEGF-C mRNA的阳性表达率明显高于正常肺组织和癌旁组织(P<0.05);不同病理类型肺癌组织中HPSE和VEGF-C蛋白阳性表达率均无显著性差异(P>0.05);不同分化程度肺癌组织中HPSE和VEGF-C蛋白阳性表达率均无显著性差异(P>0.05);Ⅲ期和Ⅳ期肺癌组织中HPSE和VEGF-C蛋白阳性表达率明显高于Ⅰ期和Ⅱ期(P<0.05);生存3年以下的肺癌组织中HPSE和VEGF-C蛋白阳性表达率明显高于生存3年以上者(P<0.05)。结论肺癌的发生、发展可能与HPSE促血管生成和VEGF-C促淋巴管生成的协同作用有关;HPSE和VEGF-C基因表达可作为判断肺癌生物学行为和患者预后的一个参考指标。Objective To study the expressions of HPSE and mRNA with invasiveness and metastasis in human lung cancer.Methods Expressions of HPSE and VEGF-C mRNA in 65 cases of lung cancer,adjacent tissues of cancer and normal tissues were tested by RT-PCR and analyzed by clinicopathological characteristics and prognosis of lung cancer.Results The positive rate of HPSE and VEGF-C mRNA in tumor tissues were significantly higher than those in adjacent tissues of cancer and normal tissues（P0.05）.There was not significant difference between HPSE and VEGF-C mRNA expressions and the type and grade of the tumor（P0.05）.But the expressions of HPSE and VEGF-C mRNA were correlated with the clinical stage and survival time of the patients（P0.05）.Conclusions Overexpressions of HPSE and VEGF-C mRNA in lung cancer tissues play role in occurrence and development of lung cancer.The expression of HPSE and VEGF-C mRNA can be used as an useful marker of the biological behavior of lung cancer and an independent prognosis factor for the patients with lung cancer.

关 键 词：肺肿瘤 肝素裂合酶 血管生长因子 逆转录-聚合酶链反应 

分 类 号：R734.2[医药卫生—肿瘤]