血链球菌胞内蛋白与胞外蛋白对中间普氏菌与牙龈卟啉单胞菌生物膜作用的研究  

Studies of the Effects of Intracellular Proteins and Extracellular Proteins in Streptococcus Sanguis on Biofilms of Prevotella Intermedia and Porphyromonas Gingivalis

作　　者：马晟利[1] 王丹[2] 闫闯[2] 

机构地区：[1]黑龙江省医院口腔科,黑龙江哈尔滨150036 [2]佳木斯大学,黑龙江佳木斯154002

出　　处：《口腔医学研究》2012年第3期206-209,共4页Journal of Oral Science Research

基　　金：黑龙江省自然科学基金资助项目(D2007-03)

摘　　要：目的:提取血链球菌胞内蛋白与胞外蛋白,研究两者对中间普氏菌(P.i)与牙龈卟啉单胞菌(P.g)生物膜的作用。方法:通过低温超速离心,有机溶剂萃取法提取血链球菌胞外蛋白;通过超声破碎,硫酸铵盐析,SephadexG-25除盐及透析的方法提取血链球菌胞内蛋白。观察血链球菌胞内蛋白与胞外蛋白对P.i及P.g的作用。观察血链球菌胞内蛋白与胞外蛋白对P.i及P.g混合培养形成的生物膜作用。结果:血链球菌胞内蛋白对混合培养的P.i与P.g的生长有明显抑制作用,其最小抑菌浓度为0.125g/L;血链球菌胞外蛋白对P.i与P.g未见抑菌作用。P.i与P.g混合培养形成的生物膜在血链球菌胞内蛋白作用后,生物膜活性降低,与对照组相比具有显著差异(P<0.05);但在血链球菌胞外蛋白作用后,生物膜活性与对照组相比差异不具有统计学意义。结论:血链球菌胞内蛋白对P.i和P.g及两者混合培养形成的生物膜具有明显抑制作用;而血链球菌胞外蛋白对P.i和P.g及两者混合培养形成的生物膜无抑制作用。Objective： To investigate the effects of Prevotella intermedia（P, i） and Porphyromonas gingivalis（P, g） in biofilms by extract extracellular and intracellular proteins of Streptococcus sanguis（S, s）. Methods： Extract extracellular proteins of S. s by high speed centrifugation using organic solvent; by ultrasonic, ammonium sulfate salting out, Sephadex G-25 column desalting through dialysis to extract intracellular proteins. Observe the effects of the intracellular proteins and extracellular proteins of S. s on biofilms of P. i and P. g. Results： The growth of P. i and P. g is significantly inhibited by intracellular proteins of S. s. The MIC of intracellular proteins on mixed P. i and P. g is 0. 125g/L;there are no significant effect of extracellular proteins of S. s on P. i and P.g. Intracellular proteins of S. s role in the biofilms of P. i and P. g mixed,the biofilms activity was decreased,compared with the control group have significant differences（P〈0.05） ; however, which roled of the extracellular proteins of S. s,the difference was not statistically significant which on the activity of biofilm compared with the control group. Conclusion： P. i, P. g and the mixed bacteria in biofilms which roled of intracellular proteins of S. s have significantly inhibition effect; there are no significantly inhibition effect when the extracellular proteins role of the P. i, P. g and the mixed bacteria in biofilms

关 键 词：血链球菌胞内蛋白 胞外蛋白 中间普氏菌 牙龈卟啉单胞菌 生物膜 

分 类 号：R781.5[医药卫生—口腔医学]