蚕豆种子贮藏蛋白质组分的比较研究  

Comparative Study on Seed Storage Protein of Faba Bean

作　　者：石建斌[1] 侯万伟[2,3] 刘玉皎[2,3] 

机构地区：[1]青海大学,西宁810016 [2]青海省农林科学院,西宁810016 [3]青海省高原作物种质资源创新与利用-国家重点实验室培育基地,西宁810016

出　　处：《植物遗传资源学报》2012年第2期304-307,共4页Journal of Plant Genetic Resources

基　　金：国家"973"前期专项(2010CB134408);现代农业产业技术体系建设专项(CARS-09-G4);青海省高校"135高层次人才培养工程"项目

摘　　要：以10份蚕豆品种为材料,利用SDS-PAGE方法,对其种子贮藏蛋白亚基组成进行了分析,结果表明:不同品种间的种子贮藏蛋白具有一定的差异,表现出一定的多态性;共分离出23条迁移率不同的亚基条带,13条具有多态性;利用贮藏蛋白亚基条带的信息,分析了品种间的蛋白相似度,相似度指数0.619～0.947,平均0.744;并通过聚类分析,在遗传相似系数0.8260水平上,将供试材料分为了3类,其中第2类具有较丰富的多样性,多样性指数达3.9296;共筛选出7对亲缘关系较远的杂交组合,可为蚕豆品种选育提供一定的科学依据。In order to investigate the difference of seed storage proteins in Faba bean, seed storage proteins ex- tracted from ten species of Faba bean were analyzed with SDS-PAGE. The result showed that it is different among the seed storage proteins of faba bean, a total of 23 subunits of different Rf were detected, counting 13 polymorphic protein subunits. The similarity index of seed storage proteins subunits in Faba bean is 0. 619 -0. 947, with an av- erage of 0. 744. Cluster analysis divided the accessions into three major clusters in the level of 0. 8260 GS, the sec- ond cluster showed higher polymorphism, and 7 pair Faba bean were identified as the parents.

关 键 词：蚕豆 种子贮藏蛋白 SDS-PAGE 亲缘关系 蛋白质亚基 

分 类 号：S643.6[农业科学—蔬菜学]