生长抑素与清胰汤联合治疗急性重症胰腺炎的实验研究  

The experimental study of combination of somatostatin with qingyitang in treatment of severe acute pancreatitis

作　　者：张国顺[1] 马利转 李玉林[1] 王玉兰[1] 徐晓平[1] 

机构地区：[1]华北煤炭医学院附属医院消化内科,河北唐山063000

出　　处：《现代预防医学》2012年第6期1485-1487,1490,共4页Modern Preventive Medicine

基　　金：唐山市科技攻关指令项目(10150204A-37)

摘　　要：目的观察生长抑素联合清胰汤对急性重症胰腺炎模型大鼠血淀粉酶和胰腺病理学的影响。为临床应用提供依据。方法 SD大鼠90只,体重(271.59±11.13)g,雄性,常规适应性喂养1周,实验前12h禁食,自由饮水。随机分成5组:假手术组(NC);急性重症胰腺炎(SAP)组;清胰汤治疗组(QY);生长抑素治疗组(SZ);联合治疗组(LH);每组18只。并将各组随机分成6h、12h、24h3个亚组,每个亚组6只。SAP模型组经胰胆管逆行注入5%牛磺胆酸钠(0.1ml/100g),假手术组胰胆管不穿刺、注药,仅翻动胰腺;生长抑素治疗组、清胰汤治疗组、联合治疗组的大鼠分别于造模成功即刻、5h、11h给予生长抑素(1μg/100g)背部皮下注射、清胰汤(1ml/100g)灌胃及两药联合治疗。动态观察记录各组及亚组(6h、12h、24h)Amy(淀粉酶测定速率法),并同步取胰腺组织观察其大体及光镜下病理变化情况。结果与假手术组比较,急性重症胰腺炎组各时间点血清AMY值明显升高;胰腺病理评分增加;差异有统计学意义(P﹤0.01);与SAP组比较,生长抑素治疗组、清胰汤治疗组血清AMY值降低,AMY与SAP组比较差异有统计学意义(P﹤0.01)胰腺病理评分降低差异有统计学意义(P﹤0.01)。与清胰汤治疗组、生长抑素治疗组比较,联合治疗组血清AMY降低;差异有统计学意义(P﹤0.05);胰腺病理评分降低(P﹤0.01)。结论联合治疗组血清各指标及胰腺组织病理评分与单一药物治疗组比较差异有统计学意义,提示联合治疗效果优于单一中药或西药。OBJECTIVE To investigate the effects of combination of somatostatin with qingyitang on severe acute panereatitis of SD-rats, detect serum concentratio of AMY, and observe pathological changes of pancreas. METHODS 90 male rats were col- lected and fed normally for one week, and they were 271.59±11.13g. Before 12 hours of experiment, the rats were given water. The rats were randomly divided into five groups： sham operation group （NC）, severe acute pancreatitis group （SAP）, so- matostatin treated group （SZ）, qingyitang treated group （QY）, somatostatin associate qingyitang treated group （LH SZ＋ QY） .and then eighteen rats were in each group. Each group were randomly divided into three groups, including 6 hours group, 12 hours group and 24 hours group, and 6 rats were in each group. Severe acute pancreatitis was induced by 5% sodium tanro- cholate pancreatic duct retrograde injection in SD-rats, NC group did not inject sodium tanrocholate, only push pancreas. The treatment group were subcutaneously injected with somatostatin （lμg/100g）, qingyitang perfusion （lml/100g） or combination of somatostatin with qingyitang. Immediately, 5 hours later and again 11 hours, they were injection with somatostatin. The amy- lase （AMY） and patholigical changesof pancreas changes were measured at the time of 6 hours, 12 hours and 24 hour after in- duction of SAP respectively; The synchronization time point were evaluated and graded. RESULTS Comparison with NC group, the AMY, level, the pathologic score of pancreatic in SAP group increased obvious statistically significant （P 〈 0.01）; Com- pared with SAP group, the AMY level in SZ, QY, LH group significantly decreased （P 〈 0.01）, and the ET/NO, TXB2/6- Keto-PGFla and pathologic score of pancreatic decreased sign/ficantly in SZ, QY and LH group （P 〈 0.01） ; Compared with SZ, QY group, the AMY level in LH group was lower statistically significant （P 〈 0.05）, and the general change and patho- logic score of pancreatic in LH grou

关 键 词：急性重症胰腺炎 生长抑素 清胰汤 联合治疗 实验研究 

分 类 号：R576[医药卫生—消化系统]